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MIR3191 Human MicroRNA Expression Plasmid (MI0014236)

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SKU
SC401396
Expression Plasmid of Human microRNA MIR3191
  $424.00
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: GGGGUCACCUCUCUGGCCGUCUACCUUCCACACUGACAAGGGCCGUGGGGACGUAGCUGGCCAGACAGGUGACCCC:
Mature Sequence: CUCUCUGGCCGUCUACCUUCCA:
Full Insert
>MI0014236 GGGGGGGGCAGGAAGGCATCTCCCGCGTCCCAGCCCCCCAGGCCCGCGCTCTGAAGCTGCCACAGTCTCT
GGCCTTCTGGTCTCTAGGCCTCTGCCACATCCGGAGGTGTGGCTTCCCTGTGGTTGGACACGAGAGGGCA
GGAGACCTGAGGCTTGTGATCTGGGTGGGGTCAGTCACTGTATCCCTGTGTGACCTTGGGATACTCCCTA
AGGGCGGGCCTCAGTTTCCCTGCCTGTCACATGGAAAAGGCAAAATGTGGCCCCTTTGGAAAATAGAGGT
CGTTAAGCCTGACCACCCCCGGGGTCACCTCTCTGGCCGTCTACCTTCCACACTGACAAGGGCCGTGGGG
ACGTAGCTGGCCAGACAGGTGACCCCAGAGCGGCGTCCCCAGGTACTTCTTGCTTTCTGGTGCCTGTGGA
GGAGAAGCTGAGAAATTGACTCACTAGCGATGGGATCACTAGAAATGAGGCGGGGAGCAGTGGCTGGGAA
AGAGGGGAGCAGGCCGAGACACCTCGAGGCCTGGAGGGGTGGTGGGTGCTGACCGCGCCCCTCTGCCTCT
CCAAGGTCCTCAGCCCTCGCTCTCGCTGGCGGAGCAGCACCTGGAGTCGCCCGTGCCCAGCGCCCCGGGG
GCTCTGGCGGGCGGTCCCACCCAGGCGGCCCCGGGAGTCCGCGGGG
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0014236
Locus ID 100422832
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Documents
Resources

file-alt Citations

An miRNA-mediated therapy for SCA6 blocks IRES-driven translation of the CACNA1A second cistron
Miyazaki, Du, Muramatsu et al
Sci Transl Med (2016) 8 (347), 347ra94 DOI: 10.1126/scitranslmed.aaf5660
Cited by 74
AI Summary: OriGene’s miR-3191-5p mimic (SC401396) was used in this study to investigate and demonstrate the ability of miR-3191-5p to inhibit CACNA1A IRES-driven translation, providing key evidence for its therapeutic potential in SCA6.
Snippet: Immunoprecipitation-coupled qRT-PCR We plated HEK293 cells onto 100-mm dishes and cotransfected each dish with 2.0 mg of the vector expressing the following: full-length CACNA1A-Q33–encoded FLAG-tagged peptides, IRES-a1ACT-Q33, miR-3191-5p (SC401396;OriGene), Ago1, Ago2, Ago3, Ago4, eIF4AII, and eIF4GII. Forty-eight hours after transfection, we harvested HEK293 cells for coimmunoprecipitation using Ago1-specific antibody (5 mg per sample, 9388S;Cell Signaling Technology),Ago2-specific antibody (5 mg per sample, 2897S;Cell Signaling Technology), Ago3-specific antibody (5 mg per sample, 5054S;Cell Signaling Technology), Ago4-specific antibody (5 mg per sample, 6913S;Cell Signaling Technology), eIF4A
Showing 1-1 of 1 papers.
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