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Mir485 Mouse MicroRNA Expression Plasmid (MI0003492)

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SKU
SC401092
Expression plasmid for mouse microRNA Mir485
  $424.00
In Stock*

Frequently Bought Together (1)

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Specifications
Specifications
Product Data
Species Mouse
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: ACUUGGAGAGAGGCUGGCCGUGAUGAAUUCGAUUCAUCUAAACGAGUCAUACACGGCUCUCCUCUCUUCUAGU:
Mature Sequence: AGAGGCUGGCCGUGAUGAAUUC:
Full Insert
>MI0003492 ACACTACCACAGGAGCTTCCAGAATACATTCTCTTCATGGAAACTGTGGAACCACGCCCCCTCTGGTCCA
GGAGGACTAACCAGATTCAAGGCTATCACCATCCCCCTTCAGGTAAGTGTGCCTCGGGTGAGCATGCACT
TAATGTAGGTGTATGTCACTCGGCTCGGCCCACTACCCAATGCTGTCCCACCCCTTCTTCACAGGACAGT
GGAAGCAGCCACCTTTTCGAGAGAGTCGATGTGTTCTTCACCAGTGTTCTTCGCCAGTGATCATGTGTGA
TACTTGGAGAGAGGCTGGCCGTGATGAATTCGATTCATCTAAACGAGTCATACACGGCTCTCCTCTCTTC
TAGTGTCAAACTCTGCCTCAGAGGTCTTCTCAAGCCACTACTGTTCCCAAGGTGGGTTTCTGGATGGCTA
AACCCATGTCAAGTGCCTTTATCCAATTGTATATTTCACACAGTTATCCCAGTGTCCAAGCTAACCCACA
CACGACTTCCTAGGACATTTCTGATTTTTTGGAAAGACCAGAGACACTGTTGGGAGATTCTGTTTAAATG
TTTAAACATACTGGCTGGCTTCTGTTTTGGTTAGTACCTAATTCTACCATGGAAGAACAACTAGAAGCAG
CCTTGGC
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains MI0003492, MI0004134
Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0003492
Reviews
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Documents
Resources

file-alt Citations

Rbfox3 controls the biogenesis of a subset of microRNAs
Kim, Yang, Zhu et al
Nat. Struct. Mol. Biol. (2014)
Cited by 51
AI Summary: OriGene’s MicroRNA Expression Plasmids were used as DNA templates to generate pri-miRNA sequences (pri-miR-15a, pri-miR-485, pri-miR-214, and pri-miR-150) for in vitro analyses investigating Rbfox3’s role in microRNA biogenesis.
Snippet: DNA templates containing the T7 promoter and encoding an approximately 200-nt region of pri-miRNAs were generated by PCR from MicroRNA Expression Plasmids (OriGene) with the following primer sets: 5′-TTG TAA TAC GAC TCA CTA TAG GGA AGC CTT TTC TGT AAA TTA C-3′ and 5′-GCT ATC ATA GGA GCT ATG AAT AAA AAG-3′ for ​pri-miR-15a;5′-TTG TAA TAC GAC TCA CTA TAG GGA GAG AGT CGA TGT GTT CTT C-3′ and 5′-ACA T...
Showing 1-1 of 1 papers.
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