Mir34b Mouse MicroRNA Expression Plasmid (MI0000404)

SKU
SC401007
Expression plasmid for mouse microRNA Mir34b
  $424.00
In Stock*
Specifications
Specifications
Product Data
Species Mouse
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: GUGCUCGGUUUGUAGGCAGUGUAAUUAGCUGAUUGUAGUGCGGUGCUGACAAUCACUAACUCCACUGCCAUCAAAACAAGGCAC:
Mature Sequence: AGGCAGUGUAAUUAGCUGAUUGU:
Full Insert
>MI0000404 AGCCGTGAAGAAGTTGTTGGAGGCGGGTCAGACCCGGCTTGCGGGAAGAAGGACTCGGGCCGGATGCCCT
GGGGTCCGGGCTGAGTCGGGGGTCTCGGGGGGGACGGCGGGGACAGGGGGCCTCAGCGCCTGGGGGTTCA
CCTGGGGTCGCGCGCGGCCCTGCTGGGTGTGGAGGGGCCTCGGGAGCCTGAGGCACCTCTCGCTCCGCCG
CCCTCGCCTGTCGACCGTGGCGAAAGGGGGCGAGGGGCCCGACCCTCGGGAGCTGCAGTGGCGGGCGCCC
GGTGCTCGGTTTGTAGGCAGTGTAATTAGCTGATTGTAGTGCGGTGCTGACAATCACTAACTCCACTGCC
ATCAAAACAAGGCACAGCATCACCCGCCGCCCGCGGGAGGAGAAGCCAGCGCTGCAGCCCCACTGACTCC
GCAGAAAGCCGAGGCCGGAGCCAGGATGCGTCAGCAAAGGGCCGGGAGCACGAGGTCTAAGTGCAAGTAA
CCCTCGGAGACCGGCGGCCTCCTTTAGGGACCGTTCGGCTACCTGTGTGTAAGAGCCAGAGCAAAAACGG
TTCAGTGATGTGTAAAGAGAATCGGTATACACCGGCCTTAACATAAAAAAAAAAAAAAAAAAAAAGGCCA
GGAAGAGCCCCC
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000404
Synonyms Mirn34b mmu-mir-34b
Locus ID 723849
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Documents
Resources

file-alt Citations

P53 regulation of osteoblast differentiation is mediated through specific microRNAs
Shah, Pendleton, Couture et al
Biochem Biophys Rep (2021) 25, 100920 DOI: 10.1016/j.bbrep.2021.100920
Cited by 9
AI Summary: OriGene’s pre-miR-140 and pre-miR-34b expression plasmids were used to generate stable MC3T3-E1 cell lines expressing these specific microRNAs, enabling the authors to investigate their roles in p53-mediated osteoblast differentiation.
Snippet: ism version 7.0 software (GraphPad Software, La Jolla, CA). 2.5 Creation of stable cell lines expressing miRNAs MC3T3- E1 cells were transfected with pre-miR-140 and pre-miR-34b expression plasmids (Origene) with a neomycin selection marker and single cell clones were isolated and expanded to stable cell lines. A stable control line contained a similar construct with a scrambled sequence. Cells h...
FigureFigureFigure
Showing 1-1 of 1 papers.
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