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Mir133a-1 Mouse MicroRNA Expression Plasmid (MI0000159)

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SKU
SC400764
Expression plasmid for mouse microRNA Mir133a-1
  $424.00
In Stock*

Frequently Bought Together (1)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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Specifications
Specifications
Product Data
Species Mouse
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: GCUAAAGCUGGUAAAAUGGAACCAAAUCGCCUCUUCAAUGGAUUUGGUCCCCUUCAACCAGCUGUAGC:
Mature Sequence: GCUGGUAAAAUGGAACCAAAU:
Full Insert
>MI0000159 AGACCTGAATGAACAAACCAACATGGTGATAATTTAGAAGCATTACTCTGAAACACATGATGATTTATCT
GTTCTTTCAAATGTTGGTTTTGAAAGGCTACTGTGTGCATTTAAGAACTAAGTAAAGATGCACTGATGTG
AGCTGCAAGAACAGCAGTGTAGGACATATGCCTAAACACGTGAATTTTCTGTTTAACAACTGGTACACTA
GTGTGGGAACCTCTAATACCTGTCATGCTATATTTCTACAAAAGAGCATTTAACCTGTTTGGATCCTACA
CCAGCAGCGGCAATGCTTTGCTAAAGCTGGTAAAATGGAACCAAATCGCCTCTTCAATGGATTTGGTCCC
CTTCAACCAGCTGTAGCTATGCATTGATTACACATGGACAGCCAGTGTTTTATGAGCATGAATTACATCT
CAACTTACTCAATTCATACAAACTGTAACTAAAAGCAAATACCTAGCAACTGTCAACCTTATTTGGAAGA
ACTAAACTGGATAAAGATGTTTCTTAAAAGCTTCATGAAATGTGATAAAAAGAATTTCAAATAAAGTTAT
TTATTTGTGGTTGTGGTATTAACTTTTCATTTTAATTAATAAAATGTAAAAATTTTTATAAAAGCACTTT
AAATCTTAGAAGTGACATAAACTTTTCTTCTTCTGA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000159
Synonyms Mirn133 Mirn133a-1 mir-133a-1
Locus ID 387151
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Documents
Resources

file-alt Citations

MicroRNA-133a-1 regulates inflammasome activation through uncoupling protein-2
Bandyopadhyay, Lane, Venugopal et al
Biochem. Biophys. Res. Commun. (2013)
Cited by 77
AI Summary: OriGene's pCMV-miR control and miR-133a-1 plasmids were used to overexpress microRNA-133a-1 in THP1 cells, enabling the authors to investigate its regulatory effect on inflammasome activation and UCP2 expression.
Snippet: Primary antibodies specific to NLRP3 (Enzo Life Sciences, Inc ... After 24 h, the medium was replaced by serum-free RPMI-1640 and cells were transfected with 2.5 μg/well DNA of pCMV-miR control (OriGene, Rockville, MD) and miR-133a-1 (Origene) plasmid using Lipofectamine
Showing 1-1 of 1 papers.
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