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MIR655 Human MicroRNA Expression Plasmid (MI0003677)

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SKU
SC400632
Expression plasmid for human microRNA MIR655
  $424.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: AACUAUGCAAGGAUAUUUGAGGAGAGGUUAUCCGUGUUAUGUUCGCUUCAUUCAUCAUGAAUAAUACAUGGUUAACCUCUUUUUGAAUAUCAGACUC:
Mature Sequence: AUAAUACAUGGUUAACCUCUUU:
Full Insert
>MI0003677 CGTCTTTCAGGAATCCGTGGAGGAAGCTTTCATATATTTTTAATGGAACCTGCCTTAGAAGTCTTTCTGA
GCATGGTTCCAATGTCAGGGTTTTGACTTTGGTGCCAGGGCTGCCTGGGCCCTGAGACTGAGGTACGGGA
ATGGAAGGTCAATGCCAGTACGTGTGCTTTCTTTGCAGGATGTGAACACCTCCCTGCCCCAACCCTGGGA
TTCAGCTCATCCCAAGAAGAATCTTCTGGAAGCAAAGACAACGGTGGGGAAGAGGCCATGGTTTTCGTTT
CAGAACTATGCAAGGATATTTGAGGAGAGGTTATCCGTGTTATGTTCGCTTCATTCATCATGAATAATAC
ATGGTTAACCTCTTTTTGAATATCAGACTCTGCCTCGGAGGGCCGCCCCCACAGGAGACGTGAGAGTGTG
TGCCTTGTGTGAGCTGAGAGCCCCTGCCCCGGCTCCGTGCTGCGTGAGGGAAGCTTCCTGGAAGTGAGGC
TGGTGGAGGGAAGAGCCCTGGGTTGCTTTGCTGTGTTTGCTGTGAATGCGATGTGTTTTTGCGAGTTATG
TGAGGTTCCCTCCTCTTTGAATGCCAACTTGTGCCTTCCCCCAGTGGCTGGGTCTGTGGCTTAGCCACAG
GGCTAGGTCCCTTCCTGGACCCAGTGCTTTTCAG
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0003677
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Documents
Resources

file-alt Citations

COX-2 induces oncogenic micro RNA miR655 in human breast cancer
Majumder, Dunn, Liu et al
Sci Rep (2018) 8 (1), 327 DOI: 10.1038/s41598-017-18612-3
AI Summary: OriGene’s pCMV-MIR miR655 expression plasmid and pCMV-MIR Mock vector were used to manipulate miR655 levels in breast cancer cells, enabling the study of miR655’s role in cancer progression and stem-like cell behavior.
Snippet: MCF7 and SKBR3 cells were transfected with 2 µg of either pCMV-MIR Mock vector (control) or pCMV-MIR miR655 expression plasmids (OriGene, MD) using the Amaxa Cell Line Nucleofector Kit V (Lonza, MO), according to the manufacturer’s protocol
FigureFigureFigure
Showing 1-1 of 1 papers.
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