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MIR328 Human MicroRNA Expression Plasmid (MI0000804)

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SKU
SC400341
Expression plasmid for human microRNA MIR328
  $424.00
In Stock*

Frequently Bought Together (1)

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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UGGAGUGGGGGGGCAGGAGGGGCUCAGGGAGAAAGUGCAUACAGCCCCUGGCCCUCUCUGCCCUUCCGUCCCCUG:
Mature Sequence: CUGGCCCUCUCUGCCCUUCCGU:
Full Insert
>MI0000804 AGCTCCAGGGAAGTGGGCTTCAGGGCCAGAGTGCGGGCCAGCTGCTCCCGCACCACCTGCATGACCTGGG
GCCGCCCAAGCTCAGCTCAGGGCCTAAGCTCTGTACCCAGATCAGCCCCCTGCCCTGGCCCAGGCCTCCC
AGCTCCCACCCCACCCACCTTGTCGAAGTCCTCCTGTGTAGCCCGCATCTCCTTCCAGGTCTTATTCAAC
AGCTGGATGCCCACACAGAAGAGCTCGTGGAAGCTCTGGTCTTGGCCGAAGAACATGGGTGAGAAGTCCT
GGGCTGTCTCAGAGCCTGGAGTGGGGGGGCAGGAGGGGCTCAGGGAGAAAGTGCATACAGCCCCTGGCCC
TCTCTGCCCTTCCGTCCCCTGCTCTTGGCCATGCTGTGCCCAGTCCAGGCCACTCACAGGGCTCCCCAAC
ACGGAGCAGCTCACACAGCAGCACCGTCAGCTGGATGCTGCCCCGGGCAAAGGGGCACTCGTGCTTGTCC
TCGCGGCTGCTGTTCTCCAACACAAACTGGGGGCGCAGAGAATGGGGCAAGGAGTCACAGCTAAGCCCAT
GGGGACCCTGCCTGGGGGATGCTGCCACCCCCCCACCCTACCCTGTCACCGACCCGGCTGTACGCGCTGG
GCGCGTTTCTGGAGAAGTACA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000804
Synonyms MIRN328 hsa-mir-328
Locus ID 442901
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Documents
Resources

file-alt Citations

miR-328 mediates a metabolic shift in colon cancer cells by targeting SLC2A1/GLUT1
Santasusagna, Moreno, Navarro et al
Clin Transl Oncol (2018)
AI Summary: In this study, the pMirTarget vector from OriGene was used to perform luciferase reporter assays, enabling the researchers to demonstrate that miR-328 directly targets the SLC2A1/GLUT1 gene in colon cancer cells.
Snippet: The following day, media were removed and cells were transfected with 50 ng of the pMirTarget vector (Origene), and 100 nM of the rellevant pre-miRNA or pre-miR-Negative Control#1 (Life Technolo- gies) using Lipofectamine 2000 in Opti-mem media (Life Technologies
Showing 1-1 of 1 papers.
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