MIR216B Human MicroRNA Expression Plasmid (MI0005569)

SKU
SC400280
Expression plasmid for human microRNA MIR216B
  $424.00
In Stock*
Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: GCAGACUGGAAAAUCUCUGCAGGCAAAUGUGAUGUCACUGAGGAAAUCACACACUUACCCGUAGAGAUUCUACAGUCUGACA:
Mature Sequence: AAAUCUCUGCAGGCAAAUGUGA:
Full Insert
>MI0005569 AGCCTCGTCTTTCTTCCATGACCAAGGTATACTAGTCTCATCCACTGGTTGTGGCTTGGGAAACTTCACT
TTTCATCTTTTCCTTCATTCTCTATGGAGATGTGTGGCAAAATTAGAGAGTATCCATTCTCATTTATGCA
CCTATCTGAATCCCAGCTATAACTCCTACTTCCCATCAATTTTAATCTTGAATAAATATAGTTTTAAAAT
TTAGCAAGACCAGAAAGATCACTGTAGAATGACGACAACTTTTTTCTATCTTAAGTACAACTTCAAAGTG
GCAGACTGGAAAATCTCTGCAGGCAAATGTGATGTCACTGAGGAAATCACACACTTACCCGTAGAGATTC
TACAGTCTGACATCTTCAAGCATCGAACCATTTCATCAACTATGCTTTTGTTATAAGCAGTTCTTTGGCC
TGGAATAAATCTAGCATGGCCAAAAAGCCAAGCTATAGCCCACTTACTGAACTTTAACTTCTTCTAAACC
AAGGTGACTACATGCCTCGTGATGGTTGAATCCCACTAAATTAAATTTTAAAATAAGAAGGTTGTTTGTA
ACATGGCTTAAAGCAGTTGTTGTCAAAGTTTGATTCCCAGACTAGCATCAGCAGCGTCACCTAGGAACCT
GTTAGA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0005569
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Documents
Resources

file-alt Citations

Colorectal cancer cells are resistant to anti-EGFR monoclonal antibody through adapted autophagy
Chen, Gao, Wang et al
Am J Transl Res (2016) 8 (2), 1190-6
AI Summary: OriGene products, specifically miR-216b mimics and antisense oligonucleotides (as-miR-216b), were used to experimentally alter miR-216b levels in colorectal cancer cells, helping to elucidate the role of miR-216b in resistance to anti-EGFR antibody therapy.
Snippet: Cell transfection MiRNAs mimics (miR-216b) and miRNAs anti- sense oligonucleotides (as-miR-216b) were ob- tained from Origene (Beijing, China). For a con- trol of these plasmids for modifying miR-216b levels, a plasmid carrying a null sequence (null) was ... Cite SaveSaving
Showing 1-1 of 1 papers.
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