MIR20B Human MicroRNA Expression Plasmid (MI0001519)

SKU
SC400270
Expression plasmid for human microRNA MIR20B
  $424.00
In Stock*
Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: AGUACCAAAGUGCUCAUAGUGCAGGUAGUUUUGGCAUGACUCUACUGUAGUAUGGGCACUUCCAGUACU:
Mature Sequence: CAAAGUGCUCAUAGUGCAGGUAG:
Full Insert
>MI0001519 AAGTAGTTGATGCTTTTGAGCTGCTTCTTATAATGTGTCTCTTGTGTTAAGGTGCATCTAGTGCAGTTAG
TGAAGCAGCTTAGAATCTACTGCCCTAAATGCCCCTTCTGGCACAGGCTGCCTAATATACAGCATTTTAA
AAGTATGCCTTGAGTAGTAATTTGAATAGGACACATTTCAGTGGTTTGTTTTTTGCCTTTTTATTGTTTG
TTGGGAACAGATGGTGGGGACTGTGCAGTGTACAGTTGTGTACAGAGGATAAGATTGGGTCCTAGTAGTA
CCAAAGTGCTCATAGTGCAGGTAGTTTTGGCATGACTCTACTGTAGTATGGGCACTTCCAGTACTCTTGG
ATAACAAATCTCTTGTTGATGGAGAGAATATTCAAAGACATTGCTACTTACAATTAGTTTTGCAGGTTTG
CATTTCAGCGTATATATGTATATGTGGCTGTGCAAATCCATGCAAAACTGATTGTGATAATGTGTGCTTC
CTACGTCTGTGTGAACACACCTTCATGCGTATCTCCAGCACTCATGCCCATTCATCCCTGGGTGGGGATT
TGTTGCATTACTTGTGTTCTATATAAAGTATTGCACTTGTCCCGGCCTGTGGAAGAAAGGAGGATTTT
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains MI0000075
Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0001519
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Documents
Resources

file-alt Citations

Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer
Chen, Zubovic, Yang et al
Nucleic Acids Res. (2016)
Cited by 69
AI Summary: OriGene products contributed to this study by providing miRNA expression plasmids for primary miR-20b and miR-107, which were used to investigate how Rbfox proteins regulate microRNA biogenesis.
Snippet: manufactures instructions. Forty-eight hours after transfection, cells were harvested for protein and RNA extraction. MiRNA expression plasmids for primary miR-20b and miR-107 were obtained from OriGene. Full-length Rbfox1 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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