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MIR199A1 Human MicroRNA Expression Plasmid (MI0000242)

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SKU
SC400250
Expression plasmid for human microRNA MIR199A1
  $424.00
In Stock*

Frequently Bought Together (1)

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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: GCCAACCCAGUGUUCAGACUACCUGUUCAGGAGGCUCUCAAUGUGUACAGUAGUCUGCACAUUGGUUAGGC:
Mature Sequence: CCCAGUGUUCAGACUACCUGUUC:
Full Insert
>MI0000242 GACCCCCAAAGAGTCAGACATTCCTCCTGAGCCCAGAAGCCACGATCCCAAACCCTGCCTCCTGCTCCGC
CTCCCCCACTCTTTAGGATTTCCTGAAAACCCAGAACTTTCTCCAGATGCGAGCCGGCCCCAGCCTTGCC
ACGTCAGAAGGGACAGAGCGGATCGTCTCGGGAAGAGTGGTGGTTTCCTTGGCTGCTCAGAGGTGCTGAG
GCACGGCCTGGCCTGGTGGCCCCAGCGTCTGCCTGGGGGGTTCTGCAGGATGGATAGCCGGCCCCGCCAA
CCCAGTGTTCAGACTACCTGTTCAGGAGGCTCTCAATGTGTACAGTAGTCTGCACATTGGTTAGGCTGGG
CTTGGGTGAGCGGCTCGTCGAGACAGGCCCCCCAAACTCGCCGGCAGGTGAGTGTCATTTTCCACCACCC
CGTTTCCCACTGTGGCAGAGCCTCGCATAGAAGATTCGAGGGCCTGGGTGGGAGAAGAGGCACTGGGGAA
AGGCAGAGGGAGCCCCGAGGCCGGCCGAGGGGTTGGAGGCCCTGGCCCTATCAGTCTGCCATCCCCACCC
CATGGCTGTAAATGTCTTGTTTATTTTTTAAATAAAGAGATATTGATGTCTTGTGTCTCACTGAGGCATC
TAAGAG
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000242
Synonyms MIRN199A1
Locus ID 406976
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Documents
Resources

file-alt Citations

ROR2 suppresses metastasis of prostate cancer via regulation of miR-199a-5p-PIAS3-AKT2 signaling axis
Tseng, Huang, Lin et al
Cell Death Dis (2020) 11 (5), 376 DOI: 10.1038/s41419-020-2587-9
Cited by 39
AI Summary: OriGene products, specifically the qSTAR miRNA qPCR detection system and the pCMV-ROR2 (RC215640) plasmid, were used in this study to quantify miRNA expression and to overexpress ROR2 in prostate cancer cells, enabling investigation of ROR2's role in suppressing metastasis via the miR-199a-5p-PIAS3-AKT2 signaling axis.
Snippet: …Plasmids and siRNA Expression of ROR2-Flag plasmid (pCMV-ROR2, RC215640), hsa-miR-199;The total RNA was isolated by using the Qiagen RNeasy Mini kit (Qiagen, Venlo, Netherlands), and 2 μg complementary DNA was produced by the RevertAid H Minus First Strand cDNA kit (Thermo Fisher Scientific). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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