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MIR185 Human MicroRNA Expression Plasmid (MI0000482)

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SKU
SC400214
Expression plasmid for human microRNA MIR185
  $424.00
In Stock*

Frequently Bought Together (1)

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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: AGGGGGCGAGGGAUUGGAGAGAAAGGCAGUUCCUGAUGGUCCCCUCCCCAGGGGCUGGCUUUCCUCUGGUCCUUCCCUCCCA:
Mature Sequence: UGGAGAGAAAGGCAGUUCCUGA:
Full Insert
>MI0000482 CGCCCAGATCAAGATATGGTGACCAAAAGGACAAGGACTATATCCATGGCTCCCTCCCAGAGCCTGGCAA
GTCAGCAGGCATGAGAGGGTGTTGGAATGCTGTGGTGGGCCTGTGTGGGGACCTTGTAGGACCTAGGGAA
CCTGCAGGGCTTGGCTTAGGGAGCACACAGGGGCCCAGGCAAAGGCAAGGTCACAGGTCGGGGGAGGTGA
AGCTGGCAGGGGGAGGGGGAGACCTGCTGGCTAGAGCTGGGTTGGGGGCCGGTGGGCAGTGGGCCTGGCT
CGAGCAGGGGGCGAGGGATTGGAGAGAAAGGCAGTTCCTGATGGTCCCCTCCCCAGGGGCTGGCTTTCCT
CTGGTCCTTCCCTCCCAATGACCGCGTCTTCGTCGAGGCCACAGCCCTTGGCTCTGCGCCCACACCTCCA
GTGCCAGGCTGTCCGGAGATCTGTTTATGGCCTTCCCTTGGACCATGGAGCCCTCCCTGCCACTGGTGCC
TGGAGGGCTGGTCTGCTGCCCCTGCACCCTGGCCAGCTAGGATGGTGGGGTCCTGCAGCTGAACTGGGGG
TGCCTTTCTGCCTGTCTGTCCTACGTGTATCAGTGGCCAGAACTGCCCTCATCCATGGCCTTGCCAGCAG
CTGGGCGTGCA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000482
Synonyms MIRN185 miR-185
Locus ID 406961
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Documents
Resources

file-alt Citations

Helicobacter pylori Dampens HLA-II Expression on Macrophages via the Up-Regulation of miRNAs Targeting CIITA
Codolo, Toffoletto, Chemello et al
Front Immunol (2019) 10, 2923 DOI: 10.3389/fimmu.2019.02923
Cited by 43
AI Summary: In this study, the OriGene rabbit anti-human vinculin antibody was used as a loading control in Western blot analyses to ensure accurate measurement of protein expression levels in macrophages.
Snippet: % Tween20 (Sigma-Aldrich), and antigens were revealed using a mouse anti-human HLA-II α-chain monoclonal antibody (1:3,000, clone TAL-1B5 Abcam) and a rabbit anti-human vinculin antibody (1:5,000, Origene). Blots were washed three times with TBS plus 0.1% Tween20 and incubated for 1 h at RT with horseradish peroxidase-conjugated anti-mouse IgG (Novex, Life Technologies) or anti-rabbit IgG (Mi;;....
FigureFigureFigure
WB
Showing 1-1 of 1 papers.
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