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MIR15B Human MicroRNA Expression Plasmid (MI0000438)

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SKU
SC400198
Expression plasmid for human microRNA MIR15B
  $424.00
In Stock*

Frequently Bought Together (1)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: UUGAGGCCUUAAAGUACUGUAGCAGCACAUCAUGGUUUACAUGCUACAGUCAAGAUGCGAAUCAUUAUUUGCUGCUCUAGAAAUUUAAGGAAAUUCAU:
Mature Sequence: UAGCAGCACAUCAUGGUUUACA:
Full Insert
>MI0000438 TTTTCCTCAAAACAGGAAGGGGATGATTATGAAGTCATTCCTAACAGTAATTTCTATGTATCCAGAACGG
CCTGCAGAGATAATACTTCTGTCTATCACATAAGTGGAAAGAAAAAGACATTTAAGGATGTTGGAAATCT
TCTTCGAAGCCATGGAATTGACTTGGACCATAATAGATTTTTAATTTTACAGGTAAGTTTATTAAAGACT
TCAAAGATTCTCTTATTCTTGTTACTTTTTTTTCTATAAAGCTAGGTTGGATGAATCCTACATTTTTGAG
GCCTTAAAGTACTGTAGCAGCACATCATGGTTTACATGCTACAGTCAAGATGCGAATCATTATTTGCTGC
TCTAGAAATTTAAGGAAATTCATTCAAAACTATGTTTTCATCATCAGATGTTCGTTTTATGTTTGGATGA
ACTGACATACTTGTTCCACTCTAGCAGCACGTAAATATTGGCGTAGTGAAATATATATTAAACACCAATA
TTACTGTGCTGCTTTAGTGTGACAGGGATACAGCAACTATTTTATCAATTGTTTGTATTTCCCTTTAAGG
TAACATTTTAAATGAAATGTATTATATTTTAATCTATCCTTTTCCTTTGTTTTTGTTCTTATTATCTCTT
CTGATATATAACCAAAAAATGAA
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000438
Synonyms MIRN15B miR-15b hsa-mir-15b
Locus ID 406949
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Documents
Resources

file-alt Citations

Tumor-associated genetic amplifications impact extracellular vesicle miRNA cargo and their recruitment of nerves in head and neck cancer
Restaino, Walz, Barclay et al
FASEB J (2024) 38 (13), e23803 DOI: 10.1096/fj.202400625RR
AI Summary: OriGene miRNA mimic vectors for miR-15b-5p (SC400198), miR-17-5p (SC400201), and miR-21-5p (SC400271) were used to investigate how specific miRNAs influence extracellular vesicle cargo and tumor-associated nerve recruitment in head and neck cancer cells.
Snippet: … Once cells reached 70% confluency, media was replaced with prewarmed serum and antibiotic-free media. 24 μg of miRNA mimic vectors for miR-15b-5p, miR-17-5p, and miR-21-5p (Origene, Rockvillem MD, USA, cat.no's: SC400198, SC400201, SC400271) was mixed with 500 μL of Opti-Mem and incubated at room temperature for 5 min
Figure
Showing 1-1 of 1 papers.
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