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MIR150 Human MicroRNA Expression Plasmid (MI0000479)

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SKU
SC400187
Expression plasmid for human microRNA MIR150
  $424.00
In Stock*

Frequently Bought Together (1)

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5 x 200 ul
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: CUCCCCAUGGCCCUGUCUCCCAACCCUUGUACCAGUGCUGGGCUCAGACCCUGGUACAGGCCUGGGGGACAGGGACCUGGGGAC:
Mature Sequence: UCUCCCAACCCUUGUACCAGUG:
Full Insert
>MI0000479 CGCAACCTCCTATTCCCCTCTGGGTCTCCGTCCCCTCCCTCTGGAGTCCACACTCCCTCTTTGCCCCTTG
CTGGTTCTCTACTGCCCCCAGCATAGGGTGGAGTGGGTGTGCAGTTTCTGCGACTCAGGGTGGCGTCCCC
CCAACCTGTCCCTGCCCCTTCCTGCCCTCTTTGATGCGGCCCCACTTCCTCTGGCAGGAACCCCCGCCCT
CCCTGGACCTGGGTATAAGGCAGGGACTGGGCCCACGGGGAGGCAGCGTCCCCGAGGCAGCAGCGGCAGC
GGCGGCTCCTCTCCCCATGGCCCTGTCTCCCAACCCTTGTACCAGTGCTGGGCTCAGACCCTGGTACAGG
CCTGGGGGACAGGGACCTGGGGACCCCGGCACCGGCAGGCCCCAAGGGGTGAGGTGAGCGGGCATTGGGA
CCTCCCCTCCCTGTACTCCCATCTCTGCTGCGGCTTTTATGCGTCTCTCCCCTTCGGGTCCCACATATCC
TCTGGTGCGCTCCTGCCTCACCGCCCCCACCCCATGCCTGTCGTCCCCACCTCTGTGTGATGCGCAAAGT
ACACCTGTTTCTATTGTACCTGCCTCTCGCGGTGGTCTGTGCTCTCCCCAGCTCTGCAAAACCCCTCCTC
CCCATGTGCCACAACCCTGGGCCACCGTGTGTCCTGTCCTGTT
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
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Reference Data
RefSeq MI0000479
Synonyms MIRN150 miRNA150
Locus ID 406942
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Documents
Resources

file-alt Citations

Dysregulation of microRNA biogenesis in the small intestine after ethanol and burn injury
Morris, Hammer, Cannon et al
Biochim. Biophys. Acta (2017)
Snippet: One day after plating, the cells were transfected with 250 ng of mir-150 expression plasmid (miR-150 plasmid) or PCMVMIR empty vector control (vector) from Origene (Rockville, MD) and Lipofectamine 2000 from Invitrogen (Carlsbad, CA). ... All 2 versions Cite SaveSaving
Synthesis and delivery of short, noncoding RNA by B lymphocytes
Almanza, Anufreichik, Rodvold et al
PNAS (2013)
Cited by 27
AI Summary: OriGene’s pCMV-MIRa150 plasmid was used to genetically program primary B lymphocytes for the synthesis and secretion of anti-miR-150 molecules in this study.
Snippet: Plasmid DNA ex- pressing anti-miR-150 (pCMV-MIRa150) is a 6.2-kb vector (Origene) into which an 82-bp insert, containing the coding sequence for the 22 bp corresponding to anti-miR-150 (Fig.1A), was cloned using Sgf I and Mlu I restriction sites. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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