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MIRLET7A2 Human MicroRNA Expression Plasmid (MI0000061)

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SKU
SC400002
Expression plasmid for human microRNA LET7A2
  $424.00
In Stock*

Frequently Bought Together (1)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
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Specifications
Specifications
Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequences
Precursor: AGGUUGAGGUAGUAGGUUGUAUAGUUUAGAAUUACAUCAAGGGAGAUAACUGUACAGCCUCCUAGCUUUCCU:
Mature Sequence: UGAGGUAGUAGGUUGUAUAGUU:
Full Insert
>MI0000061 TCGTCAACAGATATCAGAAGGCATTAAATTGTTCAGAATCACCATTATGTAACATTACTATAATTTCCAT
GCACAAATAGTTCAAGAAGGCCAGTCCCAAGTATTTGCTCCCTTCATGTTTTCATGGTAATCTCAAGCCA
AGGTTTTTGTGTTATCTCTTCTATCATCAGACTCTTTGATATGTTGTCTCTTATGAATGGCCCAAATAGG
TGACAGCACGATGAATCATTATAAGACTAACTTGTAATTTCCCTGCTTAAGAAATGGTAGTTTTCCAGCC
ATTGTGACTGCATGCTCCCAGGTTGAGGTAGTAGGTTGTATAGTTTAGAATTACATCAAGGGAGATAACT
GTACAGCCTCCTAGCTTTCCTTGGGTCTTGCACTAAACAACATGGTGAGAACGATCATGATTCCTCCAGG
CCTTTTCTCCCTATGAAAGGTAAGATTGGGTACGATTATTTTATGGTATTTTAGAATTCAAGTTTTGTTT
AATCCTTGTACCCATGCTGTATTTTCATATTCTGTTTCCTCGGGTTAGATTTTGTTTTAAGAAAAATGTG
AGGCTTTGAGGGATTCAGTATATCAGGTGTCCATTGTCACAAAAATGCTGCATAATAAACACCAAATTGT
CACAAAAAATGCAGCATT
Components 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Shipping Ambient
Reference Data
RefSeq MI0000061
Synonyms LET7A2 let-7a-2 MIRNLET7A2
Locus ID 406882
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Documents
Resources

file-alt Citations

Pre-miRNA Hsa-Let-7a-2: a Novel Intracellular Partner of Angiotensin II Type 2 Receptor Negatively Regulating its Signals
Liu, Chen, Li et al
Int J Biol Sci (2022) 18 (8), 3237-3250 DOI: 10.7150/ijbs.70455
Cited by 1
AI Summary: OriGene products, including pCMV-MIRLET7A1 (Cat. SC400001), pCMV-MIRLET7A2 (Cat. SC400002), pCMV-MIRLET7A3 (Cat. SC400003), pCMV6-AGTR2 (Cat. RC223317), and pCMV6-AGTR1 (Cat. RC209773), were used to express specific pre-miRNAs and angiotensin II receptor constructs in HEK293 cells to investigate the interaction between hsa-let-7a-2 and AGTR2 signaling.
Snippet: a humidified 5% CO2 atmosphere. pCMV-MIRLET7A1 (Cat. SC400001), pCMV-MIRLET7A2 (Cat. SC400002), pCMV-MIRLET7A3 (Cat. SC400003), pCMV6-AGTR2 (Cat. RC223317), and pCMV6-AGTR1 (Cat. RC209773) were from Origene (Rockville, MD, USA). AGTR2-GFP and AGTR1-GFP fusion plasmids were constructed from the above plasmids. Transfections and co-transfections of plasmids into HEK293 cells were performed with Lip
FigureFigureFigure
Showing 1-1 of 1 papers.
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