T7 Escherichia phage T7 Recombinant Protein

SKU TP790220

Purified recombinant protein of T7 RNA polymerase(T7), (Ala47, Gly884), 50ug

Product Data

• Species: Escherichia phage T7
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  1 MAHHHHHHMN TINIAKNDFS DIELAAIPFN TLADHYGERL AREQLALEHE SYEMAEARFR; 61 KMFERQLKAG EVADNAAAKP LITTLLPKMI ARINDWFEEV KAKRGKRPTA FQFLQEIKPE; 121 AVAYITIKTT LACLTSADNT TVQAVASAIG RAIEDEARFG RIRDLEAKHF KKNVEEQLNK; 181 RVGHVYKKAF MQVVEADMLS KGLLGGEAWS SWHKEDSIHV GVRCIEMLIE STGMVSLHRQ; 241 NAGVVGQDSE TIELAPEYAE AIATRAGALA GISPMFQPCV VPPKPWTGIT GGGYWANGRR; 301 PLALVRTHSK KALMRYEDVY MPEVYKAINI AQNTAWKINK KVLAVANVIT KWKHCPVEDI; 361 PAIEREELPM KPEDIDMNPE ALTAWKRAAA AVYRKDKARK SRRISLEFML EQANKFANHK; 421 AIWFPYNMDW RGRVYAVSMF NPQGNDMTKG LLTLAKGKPI GKEGYYWLKI HGANCAGVDK; 481 VPFPERIKFI EENHENIMAC AKSPLENTWW AEQDSPFCFL AFCFEYAGVQ HHGLSYNCSL; 541 PLAFDGSCSG IQHFSAMLRD EVGGRAVNLL PSETVQDIYG IVAKKVNEIL QADAINGTDN; 601 EVVTVTDENT GEISEKVKLG TKALAGQWLA YGVTRSVTKR SVMTLAYGSK EFGFRQQVLE; 661 DTIQPAIDSG KGLMFTQPNQ AAGYMAKLIW ESVSVTVVAA VEAMNWLKSA AKLLAAEVKD; 721 KKTGEILRKR CAVHWVTPDG FPVWQEYKKP IQTRLNLMFL GQFRLQPTIN TNKDSEIDAH; 781 KQESGIAPNF VHSQDGSHLR KTVVWAHEKY GIESFALIHD SFGTIPADAA NLFKAVRETM; 841 VDTYESCDVL ADFYDQFADQ LHESQLDKMP ALPAKGNLNL RDILESDFAF AG
• Tag: N-His
• Predicted MW: 99.9 kDa
• Concentration: >0.05 ug/ul as determined by microplate BCA method
• Purity: > 95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50mM Tris-HCl (25℃, pH7.9), 100mM NaCl, 1mM DTT, 1mM EDTA, 50% (v/v) Glycerol, 0.1% (w/v) Triton X-100
• Endotoxin: ＜10 EU/mg
• Storage: Store at -20°C after receiving vials.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_041960
• Locus ID: 1261050