tdTomato Escherichia coli Recombinant Protein

SKU
TP790045
Purified fluorescent protein tdTomato, with N-terminal HIS tag, expressed in E. coli, 250ug
  $160.00
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Specifications
Specifications
Product Data
Species Escherichia coli
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MVSKGEEVIK EFMRFKVRME GSMNGHEFEI EGEGEGRPYE GTQTAKLKVT KGGPLPFAWD ILSPQFMYGS KAYVKHPADI PDYKKLSFPE GFKWERVMNF EDGGLVTVTQ DSSLQDGTLI YKVKMRGTNF PPDGPVMQKK TMGWEASTER LYPRDGVLKG EIHQALKLKD GGHYLVEFKT IYMAKKPVQL PGYYYVDTKL DITSHNEDYT IVEQYERSEG RHHLFLGHGT GSTGSGSSGT ASSEDNNMAV IKEFMRFKVR MEGSMNGHEF EIEGEGEGRP YEGTQTAKLK VTKGGPLPFA WDILSPQFMY GSKAYVKHPA DIPDYKKLSF PEGFKWERVM NFEDGGLVTV TQDSSLQDGT LIYKVKMRGT NFPPDGPVMQ KKTMGWEAST ERLYPRDGVL KGEIHQALKL KDGGHYLVEF KTIYMAKKPV QLPGYYYVDT KLDITSHNED YTIVEQYERS EGRHHLFLYG MDELYK
Tag N-His
Predicted MW 54.2 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer PBS, pH 7.4, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq AHN10092
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Documents
Resources

file-alt Citations

Long-read sequencing quantifies synthetic mRNA abundance, integrity and host response in vivo
McLeod, Yuen, Chen et al
bioRxiv (2026)
AI Summary: OriGene’s tdTomato protein (Cat# TP790045) was used as a spike-in control in tissue samples to generate standard curves for quantifying synthetic mRNA and protein abundance in the study.
Snippet: Blank tissue samples from C57BL/6J mice were weighed and spiked with known amount of Cre recombinase (New England Biolabs;Cat# M0298) and tdTomato protein (Origene;Cat#TP790045) prior to sonication and were used to provide standard curves. For Cre recombinase ELISA, Nunc-Immuno MicroWell
Lineage tracing reveals photoreceptor precursor cell subpopulations that contribute to murine retinogenesis
Yano, Wei, Gajjar et al
Front Cell Dev Biol (2026) 14, 1814134 DOI: 10.3389/fcell.2026.1814134
AI Summary: OriGene’s His-tagged recombinant tdTomato protein (TP790045) was used to prepare compensation beads for flow cytometry analysis, aiding in the accurate identification of photoreceptor precursor cell subpopulations in the study.
Snippet: with each antibody. tdTomato compensation beads were prepared by conjugating beads with anti-His antibody (1:500, AB_914704, GenScript) and His-tagged recombinant tdTomato protein (1 μg, TP790045, OriGene). GFP BrightComp eBeads Compensation Bead (A10514, Invitrogen) were also used for the Dll1-Cre;Ai9 sample analysis to account for the EGFP reporter. Samples were
The Role of Matrix Viscoelasticity as a Biophysical Cue in Macrophage Phenotype Regulation in the Early Fracture Hematoma
Knecht
DepositOnce (2026)
AI Summary: OriGene’s tdTomato protein (#TP790045-OR) was used to incubate alginate hydrogels, enabling fluorescent labeling for visualization and analysis of the engineered extracellular matrix in macrophage phenotype studies.
Snippet: Bone fracture healing begins with the formation of a hematoma, a dynamic injury niche characterized by complex, intertwined inflammatory processes that set the trajectory;Alginate hydrogels were incubated in either 60 μg/mL green fluorescent protein (GFP) (Merck Millipore, #14-392) or 30 μg/mL tdTomato (OriGene, #TP790045-OR) in a 96-well plate for 24 hours at room temperature under light-protected conditions
Development of a high-yield Rabbit line for enhanced animal pharming
Song, Yang, Kong et al
Biol Res (2025) 58 (1), 73 DOI: 10.1186/s40659-025-00653-y
AI Summary: OriGene’s recombinant tdTomato protein (TP790045) was used to generate a standard curve for accurately quantifying tdTomato expression levels in rabbit milk in this study.
Snippet: 1/R1 are shown below: L1: 5′- TGGGCCCAGGAGATGACAGTTTT -3′ R1: 5′- GCCGGGTAGAGCTTTGGGTTTTT -3′ Determination of the concentrations of tdTomato in Rabbit milk Recombinant tdTomato (TP790045, Origene, Rockville, MD, USA) was utilized to create a standard curve. Briefly, the recombinant tdTomato was serially diluted to concentrations of 0 (no tdTomato), 0.135, 0.108, 0.054, 0.027, and 0.013
FigureFigureFigure
Photobleaching Analysis of Fluorescent Proteins in Two-Photon Microscopy at High Repetition Rates
Weng, Han
Optics Communications (2024)
Cited by 3
AI Summary: OriGene products EGFP (TP790050) and tdTomato (TP790045) were used as fluorescent protein stock solutions to investigate photobleaching behavior in two-photon microscopy experiments.
Snippet: The stock solutions of EGFP (TP790050, OriGene), tdTomato (TP790045, OriGene), Alexa Fluor 488 (A20000, ThermoFisher) and Oregon Green 488 BAPTA-1 (O6806, ThermoFisher) were diluted with a 90% glycerol (49767, Sigma) in 1× phosphate buffered saline (PBS, pH 7.4, AM9625, ThermoFisher)
MicroRNA-7a2 regulates prolactin in developing lactotrophs and prolactinoma cells
LaPierre, Godbersen, Esteban et al
Endocrinology (2020)
Cited by 15
AI Summary: OriGene’s Goat anti-RFP antibody (AB1140-100) was used in this study to detect tdTomato, facilitating the identification of specific cell populations during the investigation of miR-7a2’s role in prolactin regulation.
Snippet: Rabbit anti-ACTH (ab74976) 42 was purchased from Abcam. Goat anti-RFP (AB1140-100) 43 was used to detect tdTomato and was purchased from Origene. Mouse anti-TH (sc-25269) 44 was purchased from Santa Cruz. Donkey
Showing 1-6 of 7 papers.
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