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TEV protease Tobacco etch virus Recombinant Protein

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SKU
TP790024
Purified recombinant protein of TEV protease, with N-terminal HIS tag, expressed in E.Coli
Size:
1000 U
  $270.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Tobacco etch virus
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MHHHHHHMGE SLFKGPRDYN PISSTICHLT NESDGHTTSL YGIGFGPFII TNKHLFRRNN
GTLLVQSLHG VFKVKNTTTL QQHLIDGRDM IIIRMPKDFP PFPQKLKFRE PQREERICLV
TTNFQTKSMS SMVSDTSCTF PSSDGIFWKH WIQTKDGQCG SPLVSTRDGF IVGIHSASNF
TNTNNYFTSV PKNFMELLTN QEAQQWVSGW RLNADSVLWG GHKVFMVKPE EPFQPVKEAT
QLMN
Tag N-HIS
Predicted MW 27.8KDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 5 mM DTT, 50% glycerol
Bioactivity 1 unit is defined as the amount of enzyme required to cleave 3 µg of a substrate protein to 85% completion in 1 hours at 30°C.
Endotoxin < 1 EU per 1 µg of the protein by the LAL
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -20°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
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Documents
Resources

file-alt Citations

MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface
Houlard, Cutts, Shamim et al
Elife (2021) 10 DOI: 10.7554/eLife.73348
AI Summary: In this study, OriGene's pCMV6-MCPH1 expression plasmid and MCPH1 shRNA lentiviral particles were used to manipulate MCPH1 levels in cells, enabling the authors to investigate how MCPH1 regulates condensin II activity during interphase.
FigureFigureFigure
Acute Effects of Heat-Not-Burn, Electronic Vaping, and Traditional Tobacco Combustion Cigarettes: The Sapienza University of Rome-Vascular Assessment of Proatherosclerotic Effects of Smoking ( SUR - VAPES ) 2 Randomized Trial
Biondi-Zoccai, Sciarretta, Bullen et al
J Am Heart Assoc (2019) 8 (6), e010455 DOI: 10.1161/JAHA.118.010455
AI Summary: OriGene's commercial cotinine immunoassay (OriGene, Rockville, MD) was used in this study to measure serum cotinine levels as a biomarker of tobacco exposure in participants.
Snippet: ere 5.6% and 7.5%, respectively. Serum levels of cotinine, a metabolite of nicotine and the primary biomarker for the determination of tobacco exposure,22 were evaluated by a commercial immunoassay (Origene, Rockville, MD), and values are expressed as ng/mL;intra‐ and interassay coefficients of variation were;Serum levels of cotinine, a metabolite of nicotine and the primary biomarker for the det...
A simple method for the determination of reduction potentials in heme proteins
Efimov, Parkin, Millett et al
FEBS Lett. (2014)
Cited by 57
AI Summary: OriGene contributed to this study by providing the cDNA clones for human NPAS2 and human CLOCK, which were essential for expressing and analyzing these heme proteins.
Snippet: coli expression vector separately (pLEICS-03), using cDNA clone (Origene).The sequencing of the hNPAS2 and hCLOCK clones was carried out by the Protein and Nucleic Acids Sequencing Laboratory (University of Leicester) and verified using an Applied Biosystems ... Cite SaveSaving
Showing 1-3 of 3 papers.
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