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E74 like factor 1 (ELF1) (NM_172373) Human Recombinant Protein

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SKU
TP760629
Purified recombinant protein of Human E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
Size:
50 ug
  $270.00
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
A DNA sequence encoding human full-length ELF1
Tag N-His
Predicted MW 67.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Bioactivity EMSA assay (PMID: 26553150)
EMSA reaction positive control (PMID: 28108517)
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_758961
Locus ID 1997
UniProt ID P32519
Cytogenetics 13q14.11
RefSeq Size 3760
RefSeq ORF 1857
Synonyms EFTUD1; RIA1
Summary This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2009
Protein Categories Cytokines, Intracellular Proteins, Transciption Factors
Protein Families Transcription Factors
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Documents
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Other "E74 like factor 1" proteins (2)
SKU Description Size Price
LC428838 Lyophilized ELF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY428838 Transient overexpression lysate of E74-like factor 1 (ets domain transcription factor) (ELF1), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

SDHD promoter mutations ablate GABP transcription factor binding in melanoma
Zhang, Xu, Makowski et al
Cancer Res. (2017) 77 (7), 1649-1661 DOI: 10.1158/0008-5472.CAN-16-0919
AI Summary: In this study, the OriGene product ELF1 (TP760629) was used to analyze transcription factor binding in experiments investigating how SDHD promoter mutations affect gene regulation in melanoma.
Snippet: analysis with recombinant human GABP (Abnova, GABPA: H00002551- P01, GABPB: H00002553-P01) or ELF1 (Origene, TP760629) as described previously (23). For GABP experiments, GABPA and GABPB were mixed at ... Cite SaveSaving
An interaction proteomics survey of transcription factor binding at recurrent TERT promoter mutations
Makowski, Willems, Fang et al
Proteomics (2015)
Cited by 64
AI Summary: OriGene’s recombinant human ELF1 protein (TP760629) was used in band-shift experiments to study transcription factor binding at TERT promoter mutations in this paper.
Snippet: Band-shift experiments were performed by incubating 20 fmol of biotin labeled double stranded oligos with recombinant human ELF1 protein (Origene, TP760629) or recombinant GABPA/B (Abnova, GABPA: H00002551-P01, GABPB: H00002553-P01) in a total ... Cite SaveSaving
Showing 1-2 of 2 papers.
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