SF3B14 (SF3B6) (NM_016047) Human Recombinant Protein

SKU TP760024

Recombinant protein of human splicing factor 3B, 14 kDa subunit (SF3B14), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length SF3B14
• Tag: N-His
• Predicted MW: 14.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 100 mM arginine, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_057131
• Locus ID: 51639
• UniProt ID: Q9Y3B4
• Cytogenetics: 2p23.3
• RefSeq Size: 783
• RefSeq ORF: 375
• Synonyms: CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
• Summary: This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
• Protein Pathways: Spliceosome