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Purified recombinant protein of Lambda Protein Phosphatase (Lambda PP) produced in E. coli. Human Recombinant Protein

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SKU
TP750050
Purified recombinant protein of Lambda Protein Phosphatase (Lambda PP) produced in E. coli.
Size:
100 ug
  $500.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Tag N-terminal His
Predicted MW 25.2 KDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.1 mM MnCl2, 2 mM DTT, 50% glycerol
Bioactivity Specific activity: ~600 nmol/min/ug. Enzymatic activity was determined by measuring the amount of 4-Nitrophenol hydrolyzed from p-nitrophenyl phosphate (pNPP) at 30?.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq P03772
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Documents
Resources

file-alt Citations

Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome
Weinert, Narita, Satpathy et al
Cell (2018) 174 (1), 231-244.e12 DOI: 10.1016/j.cell.2018.04.033
AI Summary: OriGene contributed to this study by providing five recombinant purified proteins (TTC33 [TP302360], FAM192A [TP304378], TRIM33 [TP710251], NPM1 [TP303841], and JUNB [TP303595]), which were used to investigate CBP/p300-regulated acetylation dynamics.
Snippet: … Here, we combined quantitative proteomics with CBP/p300-specific catalyti.;Five of these proteins were purchased from OriGene as recombinant purified proteins (TTC33;TP302360, FAM192A;TP304378, TRIM33;TP710251, NPM1;TP303841, JUNB;TP303595) and PPM1G was purified from yeast cells, as follows. …
cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition
Padmanabhan, Rellinger, Zhu et al
Oncotarget (2017) 8 (60), 101072-101086 DOI: 10.18632/oncotarget.19557
Snippet: Gene overexpression. cFLIP short (Transcript Variant 3) plasmid was purchased from OriGene (Rockville, MD), amplified in E. coli, and purified using the QIAGEN Maxi Kit (Qiagen, Valencia, CA) per manufacturer's instructions
FigureFigureFigure
ALS-associated mutations in FUS disrupt the axonal distribution and function of SMN
Groen, Fumoto, Blokhuis et al
Hum. Mol. Genet. (2013) 22 (18), 3690-704 DOI: 10.1093/hmg/ddt222
Cited by 155
AI Summary: OriGene products contributed to this study by providing the SMN expression plasmid (OriGene, Cat# RC200446), which was used for SMN overexpression experiments to assess the rescue of axonal defects caused by mutant FUS in neurons.
Snippet: 7 Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands. ↵*To whom correspondence should be addressed at: Department of Neuroscience and Pharmacology, Rudolf Magnus Institute of Neuroscience
Showing 1-3 of 3 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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