S Protein SARS-CoV-2 Recombinant Protein

SKU
TP724025
Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal mouse Fc tag
  $770.00
4 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species SARS-CoV-2
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
S protein RBD(Arg319-Phe541) mFc(Pro99-Lys330)
Tag C-Mouse Fc Tag
Predicted MW The protein has a predicted molecular mass of 51.3 kDa after removal of the signal peptide.
Purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Preparation Affinity purification
Recommended Dilution ELISA
Storage Store the lyophilized protein at -20°C.
After reconstitution, store the protein at -80°C for 12 months.
Avoid repeated freezing and thawing.
Stability 12 months from date of despatch
Shipping Ambient
Reference Data
Locus ID 43740568
UniProt ID P0DTC2
Synonyms S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19
Summary SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Protein Families ELISA
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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