RET (NM_020975) Human Recombinant Protein

SKU TP710180

Recombinant protein of human ret proto-oncogene (RET), transcript variant 2, residues 29-635aa, with C-terminal DDK tag, expressed in sf9, 20ug

Product Data

• Species: Human
• Expression Host: Sf9
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from TrueORF clone, [RC214268], encoding the region(Met-Leu29-Arg635) of Homo sapiens RET
• Tag: C-DDK
• Predicted MW: 67.78 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, 100 mM glycine, pH 8.0, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_066124
• Locus ID: 5979
• UniProt ID: P07949
• Cytogenetics: 10q11.21
• RefSeq Size: 5629
• RefSeq ORF: 3342
• Synonyms: CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1
• Summary: This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis. [provided by RefSeq, Sep 2017]
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Endocytosis, Pathways in cancer, Thyroid cancer