SETD7 / SET9 (1-366) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 40.7 kDa (366aa) confirmed by MALDI-TOF |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 50mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 5mM DTT, 20%glycerol |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Preparation | Liquid |
| Recommended Dilution | SDS-PAGE |
| Protein Description | Recombinant human SET7/9, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_085151 |
| Locus ID | 80854 |
| UniProt ID | Q8WTS6 |
| Cytogenetics | 4q31.1 |
| Synonyms | SETD7, SET7, SET9, SET7/9, SET7/9 Histone methyltransferase, SET domain-containing protein 8, SET domain-containing protein 7 FLJ21193, SET domain-containing protein 7, Lysine N-methyltransferase 7, Lysine methyltransferase, KMT7, KIAA1717, Histone-lysine N-methyltransferase SETD7, Histone-lysine N-methyltransferase, Histone lysine N methyltransferase H3 lysine 4 specific SET7, Histone lysine methyltransferase, Histone H4-K4 methyltransferase, Histone H3-K4 methyltransferase SETD7, Histone H3 lysine 4 specific methyltransferase, Histone H3 K4 methyltransferase, H4 lysine-4 specific, H3-K4-HMTase SETD7, H3 K4 HMTase, EC 2.1.1.43 |
| Summary | Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the KR-STA-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
SetD7 (Set7/9) is a novel target of PPARγ that promotes the adaptive pancreatic β-cell glycemic response
Jetton, Flores-Bringas, Leahy et al
J Biol Chem (2021) 297 (5), 101250 DOI: 10.1016/j.jbc.2021.101250
Jetton, Flores-Bringas, Leahy et al
J Biol Chem (2021) 297 (5), 101250 DOI: 10.1016/j.jbc.2021.101250
Cited by 15
AI Summary: OriGene's PPARγ shRNA and scrambled control shRNA were used to generate stable INS-1 β-cell lines with reduced PPARγ expression, enabling the authors to demonstrate that PPARγ directly regulates SetD7 and Pdx1 expression in pancreatic β-cells.
Snippet: Rγ. We next tested direct regulation of SetD7 by PPARγ in INS-1 cells with a constitutively KD of PPARγ. We established and characterized GFP-tagged stable constitutive expression of PPARγ shRNA (Origene) and scrambled control (scr) in INS-1 β-cells (Fig. 2Ai). We obtained an shRNA clone that reduced PPARγ protein expression to 0.37-fold and correspondingly reduced Pdx1 (0.6-fold) and SetD7
SET9-Mediated Regulation of TGF-β Signaling Links Protein Methylation to Pulmonary Fibrosis
Elkouris, Kontaki, Stavropoulos et al
Cell Rep (2016) 15 (12), 2733-44 DOI: 10.1016/j.celrep.2016.05.051
Elkouris, Kontaki, Stavropoulos et al
Cell Rep (2016) 15 (12), 2733-44 DOI: 10.1016/j.celrep.2016.05.051
Cited by 75
AI Summary: OriGene products contributed to this study by providing the pRS-Set9 shRNA vector used to generate Set9-depleted cell lines and the mammalian expression vectors pCMV6-RNF111 (Arkadia), pCMV6-Smurf1, and pCMV6-Smurf2 used to study the roles of these proteins in TGF-β signaling and pulmonary fibrosis.
Lysine Methyltransferase SETD7 (SET7/9) Regulates ROS Signaling through mitochondria and NFE2L2/ARE pathway
He, Owen, Jelinsky et al
Sci Rep (2015) 5, 14368 DOI: 10.1038/srep14368
He, Owen, Jelinsky et al
Sci Rep (2015) 5, 14368 DOI: 10.1038/srep14368
Cited by 71
AI Summary: OriGene products, including DDK (FLAG)-tagged SETD7 human ORF clone, NFE2L2 human cDNA clone, pCMV6-DDK tagged empty vectors, and the Anti-DDK Magnetic Immunoprecipitation Kit, were used to express and purify proteins for studying the interaction between SETD7 and NFE2L2 in the regulation of ROS signaling.
Snippet: Plasmids and transfection. DDK (FLAG)-tagged SETD7 human ORF clone, NFE2L2 human cDNA clone and pCMV6-DDK tagged empty vectors were obtained from Origene. ... Protein pull-down was performed with Anti-DDK Magnetic Immunoprecipitation Kit (Origene ... Cite SaveSaving
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
