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UBC9 / UBE2I (1-158) Human Protein

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SKU
SA6046
Recombinant human Ubc9/UBE2I protein
Size:
100 ug
  $355.00
3 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS
Predicted MW 18 kDa (158aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 50mM HEPES buffer (pH 7.4) containing 150mM NaCl, 1mM DTT, 10%glycerol
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid protein
Recommended Dilution SDS-PAGE
Protein Description Human Ubc9 is homologous to ubiquitin-conjugating enzymes(E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1 (SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and –conjugating (E2) enzymes.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_003336
Locus ID 7329
UniProt ID P63279
Cytogenetics 16p13.3
Synonyms Ubiquitin conjugating enzyme E2 I, SUMO-conjugating enzyme UBC9, ING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBCE9
Summary The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Pathways Ubiquitin mediated proteolysis
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Documents
Resources

file-alt Citations

SUMOylation of the Kv4.2 Ternary Complex Increases Surface Expression and Current Amplitude by Reducing Internalization in HEK 293 Cells
Welch, Jansen, Baro
Front Mol Neurosci (2021) 14, 757278 DOI: 10.3389/fnmol.2021.757278
Cited by 8
AI Summary: OriGene Technologies contributed to this study by providing the pCMV6-KChIP2a (NM_145703) plasmid, which was used to co-express KChIP2a with Kv4.2 and DPP10c in HEK 293 cells to investigate the effects of SUMOylation on Kv4.2 channel surface expression and internalization.
Snippet: pCMV6-KChIP2a (NM_145703) was purchased from Origene Technologies
FigureFigureFigure
Modulation of host cell SUMOylation facilitates efficient development of Plasmodium berghei and Toxoplasma gondii
Maruthi, Singh, Reddy et al
Cell. Microbiol. (2017)
AI Summary: In this study, OriGene's p-GFP-VRS-UBE2I shRNA plasmid (TG300697) was used to silence Ubc9 expression in HepG2 cells, enabling the authors to investigate the role of SUMOylation in host-parasite interactions during Plasmodium berghei infection.
Snippet: shRNA mediated silencing of Ubc-9 expression HepG2 cells were transfected with p-GFP-VRS- UBE2I plasmid variants encoding shRNA targeting human Ubc9 mRNA (Origene TG300697). Cells were infected with P. berghei
Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response
Tomasi, Ramani, Ryoo
Am. J. Pathol. (2016) 186 (9), 2326-36 DOI: 10.1016/j.ajpath.2016.05.007
Cited by 30
AI Summary: OriGene products contributed to this study by providing the UBC9-HA tagged expression vector and the UBC9 antibody, which were used for gene overexpression and protein detection to investigate UBC9's role in inflammatory signaling.
Snippet: UBC9-HA tagged expression vector was from Origene (Rockville, MD). ... Antibodies used for Western blotting were: UBC9 (Origene), pTyr-15 CDK1/pThr-161 CDK1 and Ikbα (Novus Biologicals, Littleton, CO), and actin (Sigma). ... Cite SaveSaving
Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization
Fuhs, Insel
J. Biol. Chem. (2011) 286 (17), 14830-41 DOI: 10.1074/jbc.M110.214270
Cited by 6
AI Summary: In this study, OriGene products including mammalian expression plasmids for eNOS, SUMO-1, SUMO-3, Ubc9, PIAS3, and PIASy were used to investigate the SUMOylation of Caveolin-3 and its impact on G-protein-coupled receptor desensitization.
Snippet: The mammalian expression plasmids for eNOS, SUMO-1, SUMO-3, Ubc9, PIAS3, and PIASy were obtained from Origene (catalog nos. ... 38 appeared to be the major site of SUMOylation;however, other sites can be modified, suggesting that two or more lysines might need to be
SUMOylation regulates insulin exocytosis downstream of secretory granule docking in rodents and humans
Dai, Plummer, Casimir et al
Diabetes (2011) 60 (3), 838-47 DOI: 10.2337/db10-0440
Cited by 94
AI Summary: OriGene products contributed human SUMO1, Ubc9, and SENP1 constructs (in the pCMV6-XL4 vector), which were used to manipulate and study the SUMOylation pathway’s effects on insulin secretion and β-cell exocytosis in this research.
Snippet: The human SUMO1, Ubc9, and SENP1 constructs were in the pCMV6-XL4 vector and were from Origene Technologies (Rockville, MD). ... 2E). Some Munc18a may be pulled down with syntaxin-1A independent of the SNARE complex.
FigureFigureFigure
Showing 1-5 of 5 papers.
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