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HSPB1 / HSP27 (1-205) Human Protein

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SKU
SA6031
Recombinant human HSP27 protein
Size:
100 ug
  $355.00
3 Weeks*
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Other products for "HSPB1 or NM_001540"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK
Predicted MW 22.7 kDa (205aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM HEPES buffer (pH 7.4) containing 100mM KCl, 1mM DTT
Endotoxin < 1 EU per 1ug of protein (determined by LAL method)
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant Human Hsp27 was overexpressed in E. coli and purified by conventional chromatography.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_001531
Locus ID 3315
UniProt ID P04792
Cytogenetics 7q11.23
Synonyms Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.
Summary This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. provided by RefSeq, Aug 2017
Protein Categories Growth Factors, Intracellular Proteins, Nervous system: Neurodegenerative diseases
Protein Pathways MAPK signaling pathway, VEGF signaling pathway
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Documents
Resources

file-alt Citations

MiR-363-5p modulates regulatory T cells through STAT4-HSPB1-Notch1 axis and is associated with the immunological abnormality in Graves' disease
Yin, Ge, Ge et al
J Cell Mol Med (2021)
Cited by 7
AI Summary: OriGene’s pCMV6-HSPB1 expression vector was used in this study to investigate how overexpression of HSPB1 affects Treg cell function and the STAT4-HSPB1-Notch1 signaling axis in the context of Graves' disease.
Snippet: The miR- 363- 5p agomir, antagomir (defined as miR- 363 and anti- miR- 363 in the Figures) and negative control sequences (Ribobio) as well as pCMV6- HSPB1 (Origene) and pCMV3- STAT4 expression vectors (Sino Biological), respectively, were transfected into
Chemotherapy Sensitizes Therapy-Resistant Cells to Mild Hyperthermia by Suppressing Heat Shock Protein 27 Expression in Triple-Negative Breast Cancer
Mu, Wu, Zhou et al
Clin Cancer Res (2018) 24 (19), 4900-4912 DOI: 10.1158/1078-0432.CCR-17-3872
AI Summary: In this study, OriGene’s HSP27 (heat shock protein 27) shRNA lentiviral particles were used to knock down HSP27 expression, helping to demonstrate that suppression of HSP27 sensitizes triple-negative breast cancer cells to mild hyperthermia following chemotherapy.
FigureFigure
Detection of salivary heat shock protein 27 by enzyme-linked immunosorbent assay and its correlation with histopathology of oral leukoplakia
Bhavana, Madhura, Kumar et al
J Oral Maxillofac Pathol (2018) 22 (3), 307-313 DOI: 10.4103/jomfp.JOMFP_86_18
Snippet: ORIGENE Hsp27 ELISA kit (Sentier Laboratories Private Limited, Bengaluru) was used for the present study. At the end of the procedure, the mean absorbance and Hsp27(pg/ml) values were calculated for both control and leukoplakic samples. A curve was plotted for both the groups and the correlation of mean absorbance and mean Hsp27 were calculated for both the groups
FigureFigureFigure
microRNA-577 suppresses tumor growth and enhances chemosensitivity in colorectal cancer
Jiang, Ju, Zhang et al
J. Biochem. Mol. Toxicol. (2017)
AI Summary: OriGene’s pCMV6 Entry vector containing the human HSP27 open reading frame was used to overexpress HSP27, enabling the authors to investigate its role as a target of miR-577 in colorectal cancer cell growth and chemosensitivity.
Snippet: the pcDNA3.1(-) vector (Invitrogen). For overexpression of HSP27, the entire open reading frame of human HSP27 cloned in the pCMV6 Entry vector was obtained from Origene (Rockville, MD). For luciferase reporter assays ... All 2 versions Cite SaveSaving
Phosphorylation enhances recombinant HSP27 neuroprotection against focal cerebral ischemia in mice
Shimada, Tanaka, Shimura et al
Neuroscience (2014) 278, 113-21 DOI: 10.1016/j.neuroscience.2014.07.073
Cited by 26
AI Summary: OriGene products contributed to this study by providing the recombinant HSP27 (rHSP27) protein, which was used to assess and compare the neuroprotective effects of phosphorylated and non-phosphorylated forms of HSP27 in a mouse model of cerebral ischemia.
Mutants of human αB-crystallin cause enhanced protein aggregation and apoptosis in mammalian cells: influence of co-expression of HspB1
Raju, Abraham
Biochem. Biophys. Res. Commun. (2013) 430 (1), 107-12 DOI: 10.1016/j.bbrc.2012.11.051
Cited by 31
AI Summary: OriGene’s pCMV6-Myc-DDK-tagged-HspB1 (Hsp27) plasmid was used for co-transfection experiments to study the effect of HspB1 overexpression on protein aggregation and apoptosis in cells expressing mutant αB-crystallin.
Snippet: vector were transfected. In co-transfection experiments, 1 μg each of plasmid DNA;pCMV6-Myc-DDK-tagged-HspB1 (Hsp27) (Origene, MD) were co-transfected with YFP-tagged mutated αB-crystallin constructs. After 48 hours
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Showing 1-6 of 7 papers.
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