PTPN1 (1-321) Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 37.3 kDa (321aa) |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Presentation State | Purified |
| Buffer | Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA1mM DTT, 20% glycerol |
| Bioactivity | Specific activity is > 10,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Preparation | Liquid purified protein |
| Recommended Dilution | SDS-PAGE, Enzyme Activity |
| Protein Description | The protein coding region of the catalytic domain of PTPN1 (PTP-1B) (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques. |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| RefSeq | NP_002818.1 |
| Locus ID | 5770 |
| UniProt ID | P18031 |
| Cytogenetics | 20q13.13 |
| Synonyms | Protein tyrosine phosphatase non-receptor type 1, PTPN1, PTP1B, PTP-1B, Protein Tyrosine Phosphatase 1B, EC 3.1.3.48, Protein-tyrosine phosphatase1B, Tyrosine-protein phosphatase non-receptor type 1, Protein tyrosine phosphatase non-receptor type 1, Protein Tyrosine Phosphatase Non Receptor Type-1 |
| Summary | The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2013 |
| Protein Categories | Growth Factors, Membrane Proteins |
| Protein Families | Druggable Genome, Phosphatase, Transmembrane |
| Protein Pathways | Adherens junction, Insulin signaling pathway |
Reviews
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Resources
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Citations
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