Tumor necrosis factor (TNF-alpha) Canine Protein

CAT#: SA1002

Tumor necrosis factor (TNF-alpha) canine recombinant protein, 20 µg

Specifications

Product Data

• Species: Canine
• Expression Host: Pichia pastoris
• Predicted MW: 17.3 kDa
• Purity: >95% pure by SDS PAGE analysis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
  Buffer System: PBS.
  Further dilutions should be made in a buffer containing carrier protein.
  Preservative: None
  Stabilizer: None
• Bioactivity: Biological: Has an ED₅₀ of 10pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.
• Endotoxin: < 1.1EU/µg
• Reconstitution: Restore with 0.5 ml distilled water.
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation: Lyophilized purified protein
• Applications: Functional Assays.
• Protein Description: Recombinant Canine TNF alpha protein expressed in Pichia pastoris.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001003244
• Locus ID: 403922
• Synonyms: cTNF; TNFA
• Summary: tumor necrosis factor