Protein G Streptococcus Protein

Name: Protein G Streptococcus Protein
Brand: OEM
SKU: RA101B
Price: 505 USD
Availability: InStock

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SKU

RA101B

Protein G Biotin Conjugated

Datasheet SDS

Motors containing inactive subunits exhibit slower start times, lower packaging speeds, and more pausing. a Schematic of the optical trapping assay to measure the packaging dynamics of a single T4 motor. A multi-stream laminar flow chamber (left) is used to manipulate samples and buffer. A bead coated with anti-T4 antibodies is captured in one trap in a flow stream containing ATP (green stream; step ①). Another bead conjugated with arrested T4 capsid–gp17-DNA motors is captured in a second optical trap in the stream containing non-hydrolyzable analog ATP-γS (red stream; step ②). The motor bound to DNA in the presence of ATP-γS cannot initiate packaging due to a lack of energy source. To form a tether between the bead pair, the beads are moved close for 1 s and separated apart to detect binding of capsid to antibody (step ③). After a constant force (F = 5 pN) load is applied to the tether, the traps are moved into ATP and packaging is detected as the decrease of DNA extension when DNA is translocated into the capsid by the motor (step ④). b Extension of unpackaged DNA vs. time, under constant force load. Packaging activity is observed within seconds of entering the ATP stream. Representative packaging trajectories with WT (blue) and ISD motors (orange) are shown. c Distribution of the start times of WT (blue, n = 44) and ISD motors (orange, n = 62), defined as the dwell time between entry into the ATP stream and the start of packaging. d Distribution of mean pause-free packaging velocity derived from each packaging trace. ISD motors (orange) show a lower packaging velocity than WT motors (blue). e Histogram of the logarithm of pause frequency, defined as the number of pauses per kb packaged. ISD motors (orange) exhibit more pauses than WT motors (blue) on average. Motors with long start times (black stars; c–e) have a lower packaging velocity and higher pause frequency. The number of trajectories used is indicated by n = #. Fig 4. PMID: 34772936.

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Specifications

Specifications
Product Data
Species	Streptococcus
Concentration	1 by UV absorbance at 280 nm
Purity	Protein G Biotin Conjugated was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A.
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
Preparation	Lyophilized (sterile filtered) Ig fraction.
Recommended Dilution	ELISA,Immunohistochemistry,Western Blot
Protein Description	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A.
Note	Protein G Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.
Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability	Expiration date is one (1) year from date of receipt.
Shipping	Blue Ice
Reference Data
UniProt ID	P19909
Synonyms	ProG, Streptococcus G protein, Protein G Biotin Conjugated
Summary	Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. Protein G Biotin Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.
Protein Families	000-1/200, 000-1/40, 000-1/5, 000., 000. Immunochemistry: 1/1, 000. Western Blot: 1/10, ELISA : 1/20

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FAQs
Protein FAQs
SDS
SDS for RA101B

Resources

Recombinant Protein Resources
Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Protein G Streptococcus Protein

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Protein G Streptococcus Protein

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