Protein G Protein
Specifications
| Product Data | |
| Concentration | 1.0 mg/ml |
|---|---|
| Purity | Recombinant Protein G is chromatographically pure and shows predominantly a single band by SDS-PAGE. Protein G was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Protein G. No reaction was observed against anti-Protein A. |
| Presentation State | Aff - Purified |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Preparation | Lyophilized purified Ig fraction without preservatives. |
| Recommended Dilution | ELISA,Western Blot |
| Protein Description | Protein G (recombinant, E. coli), unconjugated. Extinction Coefficient: 1% at 280 nm is 9.5 (E0.1% of 9.5). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Protein G. No reaction was observed against anti-Protein A. |
| Note | Protein G is suitable for use as an antigen, as a control or standard in assays, for conjugation and for most other immunological methods. Protein G contains only IgG binding domains, ensuring maximum specific IgG binding. |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Protein G is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| UniProt ID | P19909 |
| Synonyms | ProG, Streptococcus G protein |
| Summary | Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. While native Protein G binds to albumin, recombinant Protein G is designed to contain only immunoglobulin binding domains to ensure the maximum specific IgG binding capacity. |
| Protein Families | as a Control or standard in assays, ensuring maximum specific IgG binding., for conjugation and for most other immunological methods. This recombinant Protein G contains only IgG binding domains, Suitable for use as an antigen |
Reviews
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Resources
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