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Protein A (Biotin conjugated) Protein

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SKU
RA100B
Protein A Biotin Conjugated
Size:
500 ug
  $250.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Concentration 1.0 mg/mL by UV absorbance at 280 nm
Purity This product was prepared from chromatographically pure Protein A.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein A.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
Preparation Lyophilized
Recommended Dilution ELISA,Immunohistochemistry,Western Blot
Protein Description Single precipitin arc against anti-biotin and anti-Protein A.
Note Protein A Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Reference Data
UniProt ID P38507
Synonyms ProA, Staphylococcus A protein, Protein A Biotin Conjugated
Summary Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies.
Protein Families 000 - 1/10, 000 - 1/5, 000., 000.
Immunohistochemistry: 1/1, 000.
Western Blot: 1/2, ELISA: 1/200
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Documents
Resources

file-alt Citations

Circulating NK cell subsets and NKT‑like cells in renal transplant recipients with acute T‑cell‑mediated renal allograft rejection
Xu, Han, Huang et al
Mol Med Rep (2019) 19 (5), 4238-4248 DOI: 10.3892/mmr.2019.10091
AI Summary: OriGene's hematoxylin (cat. no. ZLI 9608) and eosin (cat. no. ZLI 9613) staining products were used to prepare tissue slides for grading acute rejection and controls in this study of NK cell subsets in renal transplant recipients.
Snippet: All slides were stained with hematoxylin (cat. no. ZLI 9608) and eosin (cat. no. ZLI 9613;both OriGene Technologies, Inc., Beijing, China) for 3-5 min at room temperature and used for grading of acute rejection and the controls (IRI and CNI toxicity)
FigureFigureFigure
REV-ERBα reduction is associated with clinicopathological features and prognosis in human gastric cancer
Wang, Wang, Wei et al
Oncol Lett (2018) 16 (2), 1499-1506 DOI: 10.3892/ol.2018.8809
AI Summary: OriGene products, specifically the streptavidin-biotin horseradish peroxidase complex and 3,3′-diaminobenzidine, were used for immunohistochemical staining to detect protein expression in gastric cancer tissue samples in this study.
Snippet: OriGene Technologies, Inc., Beijing, China), the tissue slides were incubated with a streptavidin-biotin horseradish peroxidase complex for 30 min at room temperature, followed by incubation with 3,3′-diaminobenzidine (ZSGB-BIO;OriGene Technologies, Inc.)
FigureFigureFigure
Showing 1-2 of 2 papers.
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