Protein A (AP conjugated) Staphylococcus aureus Protein
Specifications
| Product Data | |
| Species | Staphylococcus aureus |
|---|---|
| Concentration | 1.0 mg/mL by UV absorbance at 280 nm |
| Purity | Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Preparation | Liquid (sterile filtered) Ig fraction. |
| Recommended Dilution | ELISA,Immunohistochemistry,Western Blot |
| Protein Description | Alkaline Phosphatase Conjugated Protein A. |
| Note | Protein A Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader. |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. Protein A Alkaline Phosphatase Conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Reference Data | |
| UniProt ID | P38507 |
| Synonyms | ProA, Staphylococcus A protein, ALP, Protein A Alk Phos |
| Summary | Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. |
| Protein Families | 000 of the stock concentration is suggested for this product., 500), ELISA and Immunohistochemistry (1:200- 1:1, Suitable for Western Blot (1:500- 1:2 |
Reviews
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Resources
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Citations
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