Protein A (AP conjugated) Staphylococcus aureus Protein

CAT#: RA100AP

Protein A (AP conjugated) s. aureus protein, 1 mg

Specifications

Product Data

• Species: Staphylococcus aureus
• Concentration: lot specific
• Purity: This product was prepared from chromatographically pure Protein A.
• Conjugation: AP
• Buffer: State: Liquid (sterile filtered) Ig fraction.
  Buffer System: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as Stabilizer and 0.01% (w/v) Sodium Azide as Preservative.
  Label: Alkaline Phosphatase (Calf Intestine, Molecular Weight 140, 000 daltons).
  Presentation Label: AP
• Preparation: Liquid (sterile filtered) Ig fraction.
• Applications: Suitable for Western Blot (1:500- 1:2,500), ELISA and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-avidin/biotin based enzymatic assays.
  Recommended dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product.
• Protein Description: Alkaline Phosphatase Conjugated Protein A.
• Note: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
• Storage: Store vial at 2-8°C before opening.
  DO NOT FREEZE!!
  This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Stability: Shelf life: one year from despatch.