Adiponectin (biologically active) Mouse Protein

CAT#: PA502X

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Adiponectin (biologically active) mouse protein, 0.1 mg



USD 580.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Description Adiponectin (biologically active) mouse protein, 0.1 mg
Species Mouse
Expression Host HEK293
Expression cDNA Clone or AA Sequence EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK
Concentration lot specific
Purity >98% as determined by densitometric image analysis
Buffer Presentation State: Purified
State: Lyophilized (0.4µm filtered) purified protein
Buffer System: 0.05M Phosphate buffer, 0.075M NaCl, pH 7.4
Bioactivity Biological: Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocyte. It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 µg/ml. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production.
Reconstitution Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Preparation Lyophilized (0.4µm filtered) purified protein
Applications ELISA.
Western Blot.
Cell culture and/or animal studies.
Protein Description Mouse Adiponectin
Note Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_033735
Locus ID 11450
UniProt ID Q60994
Cytogenetics 16 13.96 cM
Synonyms 30kDa; Acdc; Acrp30; Ad; Adid; adipo; apM1; APN; GBP28
Summary Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.[UniProtKB/Swiss-Prot Function]

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