Adiponectin (biologically active) Mouse Protein
Adiponectin (biologically active) mouse protein, 0.1 mg
|Expression cDNA Clone or AA Sequence||
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK
|Purity||>98% as determined by densitometric image analysis|
|Buffer||Presentation State: Purified
State: Lyophilized (0.4µm filtered) purified protein
Buffer System: 0.05M Phosphate buffer, 0.075M NaCl, pH 7.4
|Bioactivity||Biological: Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocyte. It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 µg/ml. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production.|
|Reconstitution||Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.|
|Preparation||Lyophilized (0.4µm filtered) purified protein|
Cell culture and/or animal studies.
|Protein Description||Mouse Adiponectin|
|Note||Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.|
|Storage||Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C.
Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Cytogenetics||16 13.96 cM|
|Synonyms||30kDa; Acdc; Acrp30; Ad; adipo; apM1; APN; GBP28|
|Summary||Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.[UniProtKB/Swiss-Prot Function]|
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