Adiponectin (biologically active) Mouse Protein
Specifications
| Product Data | |
| Species | Mouse |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Concentration | lot specific |
| Purity | >98% as determined by densitometric image analysis |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized (0.4µm filtered) purified protein Buffer System: 0.05M Phosphate buffer, 0.075M NaCl, pH 7.4 |
| Bioactivity | Biological: Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocyte. It can also activate AMPK in HepG2 human hepatocytes at the concentration of as low as 1.0 µg/ml. In vitro gluconeogenesis assay in primary rat hepatocytes was performed, showing the murine adiponectin derived from mammalian cells can inhibit glucose production. |
| Reconstitution Method | Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. |
| Preparation | Lyophilized (0.4µm filtered) purified protein |
| Recommended Dilution | ELISA. Western Blot. Cell culture and/or animal studies. |
| Protein Description | Mouse Adiponectin |
| Note | Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_033735 |
| Locus ID | 11450 |
| UniProt ID | Q60994 |
| Cytogenetics | 16 13.96 cM |
| Synonyms | ADIPOQ, ACDC, ACRP30, APM1, GBP28 |
| Summary | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.UniProtKB/Swiss-Prot Function |
| Protein Categories | Cytokines, Growth Factors, Secreated Proteins |
| Protein Families | ELISA. Western Blot. Cell culture and/or animal studies. |
Reviews
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Resources
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Citations
Involvement of endothelial apoptosis underlying chronic obstructive pulmonary disease-like phenotype in adiponectin-null mice: implications for therapy
Nakanishi, Takeda, Tetsumoto et al
Am J Respir Crit Care Med (2011) 183 (9), 1164-75 DOI: 10.1164/rccm.201007-1091OC
Nakanishi, Takeda, Tetsumoto et al
Am J Respir Crit Care Med (2011) 183 (9), 1164-75 DOI: 10.1164/rccm.201007-1091OC
AI Summary: OriGene products contributed to this study by providing the mouse Adiponectin ELISA kit (Cat# EA100773), which was used to measure plasma adiponectin levels and assess changes in adiponectin expression in the experimental models.
Expanding the adipokine network in cartilage: identification and regulation of novel factors in human and murine chondrocytes
Conde, Gomez, Bianco et al
Ann Rheum Dis (2011) 70 (3), 551-9 DOI: 10.1136/ard.2010.132399
Conde, Gomez, Bianco et al
Ann Rheum Dis (2011) 70 (3), 551-9 DOI: 10.1136/ard.2010.132399
Cited by 127
AI Summary: In this study, OriGene products—including human chemerin cDNA (RC217706), human LCN2 cDNA (RC221547), and human SAA3 cDNA (RC221888)—were used as positive controls in PCR and western blot experiments to assess the expression and regulation of novel adipokines in human and murine chondrocytes.
Adiponectin deficiency increases allergic airway inflammation and pulmonary vascular remodeling
Medoff, Okamoto, Leyton et al
Am J Respir Cell Mol Biol (2009) 41 (4), 397-406 DOI: 10.1165/rcmb.2008-0415OC
Medoff, Okamoto, Leyton et al
Am J Respir Cell Mol Biol (2009) 41 (4), 397-406 DOI: 10.1165/rcmb.2008-0415OC
AI Summary: In this paper, OriGene’s mouse Adiponectin (APN) ELISA kit was used to measure adiponectin levels, helping to assess the relationship between APN deficiency and increased allergic airway inflammation and pulmonary vascular remodeling in mice.
Adiponectin deficiency promotes endothelial activation and profoundly exacerbates sepsis-related mortality
Teoh, Quan, Bang et al
Am J Physiol Endocrinol Metab (2008) 295 (3), E658-64 DOI: 10.1152/ajpendo.90384.2008
Teoh, Quan, Bang et al
Am J Physiol Endocrinol Metab (2008) 295 (3), E658-64 DOI: 10.1152/ajpendo.90384.2008
Cited by 91
AI Summary: OriGene products contributed to this study by providing recombinant C39A trimeric adiponectin, which was used to assess the specific effects of adiponectin oligomer forms on endothelial adhesion molecule expression in sepsis models.
Adiponectin modulates the glycogen synthase kinase-3beta/beta-catenin signaling pathway and attenuates mammary tumorigenesis of MDA-MB-231 cells in nude mice
Wang, Lam, Lam et al
Cancer Res (2006) 66 (23), 11462-70 DOI: 10.1158/0008-5472.CAN-06-1969
Wang, Lam, Lam et al
Cancer Res (2006) 66 (23), 11462-70 DOI: 10.1158/0008-5472.CAN-06-1969
AI Summary: In this study, OriGene’s human Adiponectin cDNA clone and Adiponectin adenoviral expression vector were used to overexpress adiponectin in breast cancer cells and in vivo models, enabling the authors to demonstrate adiponectin’s inhibitory effects on tumor growth and its modulation of the GSK-3β/β-catenin signaling pathway.
Adiponectin attenuates allergen-induced airway inflammation and hyperresponsiveness in mice
Shore, Terry, Flynt et al
J Allergy Clin Immunol (2006) 118 (2), 389-95 DOI: 10.1016/j.jaci.2006.04.021
Shore, Terry, Flynt et al
J Allergy Clin Immunol (2006) 118 (2), 389-95 DOI: 10.1016/j.jaci.2006.04.021
Cited by 328
AI Summary: In this study, OriGene’s quantitative real-time PCR primers for adiponectin, adiponectin receptor 1, adiponectin receptor 2, and T-cadherin were used to measure gene expression levels in mouse tissues, helping to demonstrate how adiponectin and its receptors are regulated during allergen-induced airway inflammation.
Showing 1-6 of 9 papers.
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