PDGFA (PDGF-AB) Human Protein

CAT#: DA3552

PDGFA (PDGF-AB) human recombinant protein, 5 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 25.5 kDa
• Purity: >95% by SDS-PAGE and visualised by silver stain
• Presentation: Purified
• Buffer: 50 mM Acetic Acid
• Bioactivity: Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml.
  Specific: > 1 x 10e6 units/mg.
• Endotoxin: < 0.1 ng per ug of PDGF-AB
• Reconstitution: Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50mM acetic acid to a concentration not lower than 100μg/ml. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA; 0.1%) is recommended.
• Preparation: Lyophilized purified fraction without stabilizer
• Protein Description: Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
• Note: Always centrifuge product before opening vial!
• Storage: Store The lyophilized PDGF-AB for one month at room temperature or -20°C. Reconstituted PDGF-AB should be stored in working aliquots at -20°C to -70°C.
  Avoid repeated freeze-thaw cycles!
• Stability: Shelf life: One year from despatch.

Reference Data

• RefSeq: NP_002598
• Locus ID: 5154
• UniProt ID: P04085
• Cytogenetics: 7p22.3
• Synonyms: PDGF-A; PDGF1
• Summary: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton