PDGFA (PDGF-AA) Human Protein

CAT#: DA3551X

PDGFA (PDGF-AA) human recombinant protein, 20 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 28.5 kDa
• Purity: >95% pure by SDS-PAGE and visualised by silver stain.
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified fraction.
  Buffer System: 50 mM Acetic Acid
• Bioactivity: Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml.
  Specific: > 1 x 10e6 units/mg.
• Endotoxin: < 0.1 ng per µg of PDGF-AA.
• Preparation: Lyophilized purified fraction.
• Protein Description: Recombinant Human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Range: 0.2-10.0 ng/ml
• Note: Always centrifuge product before opening vial!
• Storage: Store lyophilized human PDGF-AA at -20°C.
  Reconstituted PDGF-AA should be stored in working aliquots at -20°C to -70°C.
  Avoid repeated freeze-thaw cycles!
• Stability: Shelf life: One year from despatch.

Reference Data

• RefSeq: NP_002598
• Locus ID: 5154
• UniProt ID: P04085
• Cytogenetics: 7p22.3
• Synonyms: PDGF-A; PDGF1
• Summary: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton