PDGFA (PDGF-AA) Human Protein
|Description||PDGFA (PDGF-AA) human recombinant protein, 2 µg|
|Expression Host||E. coli|
|Predicted MW||28.5 kDa|
|Purity||>95% pure by SDS-PAGE and visualised by silver stain.|
|Buffer||Presentation State: Purified
State: Lyophilized purified fraction.
Buffer System: 50 mM Acetic Acid
|Bioactivity||Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml.
Specific: > 1 x 10e6 units/mg.
|Endotoxin||< 0.1 ng per µg of PDGF-AA.|
|Preparation||Lyophilized purified fraction.|
|Protein Description||Recombinant Human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Range: 0.2-10.0 ng/ml|
|Note||Always centrifuge product before opening vial!|
|Storage||Store lyophilized human PDGF-AA at -20°C.
Reconstituted PDGF-AA should be stored in working aliquots at -20°C to -70°C.
Avoid repeated freeze-thaw cycles!
|Stability||Shelf life: One year from despatch.|
|Summary||This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]|
|Protein Families||Druggable Genome|
|Protein Pathways||Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
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