KITLG / SCF Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Predicted MW | 18.5 kDa |
| Purity | >98% > 98% by RP-HPLC, Anion-exchange FPLC, Silver stain |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized without stabilizer Buffer System: 10 mM acetic acid |
| Bioactivity | Biological: Recombinant human SCF is fully biologically active when compared to standards. The ED50 determined by the dose-dependent stimulation of human TF-1 cells is < 2.0 ng/ml. Specific: 1 x 10e5 units/mg |
| Endotoxin | < 0.1 ng per µg (IEU/µg) of rh SCF |
| Reconstitution Method | The lyophilized powder should be reconstituted in water to a concentration not less than 0.1 mg/ml. This solution can be stored at 4°C for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum. |
| Preparation | Lyophilized without stabilizer |
| Protein Description | Recombinant Human SCF |
| Note | Centrifuge vials before opening! |
| Storage | Prior and following reconstitution store (in aliquots) at 2 - 8 °C for up to two weeks or -20 °C for longer. For long term storage add a carrier protein (0.1 % HAS or BSA). Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_000890 |
| Locus ID | 4254 |
| UniProt ID | P21583 |
| Cytogenetics | 12q21.32 |
| Synonyms | KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF |
| Summary | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008 |
| Protein Categories | Cytokines, Growth Factors, Intracellular Proteins, Membrane Proteins, Nervous system Diseases |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer |
Reviews
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Resources
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Citations
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