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Interleukin-4 / IL4 Human Protein

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SKU
DA3546
Interleukin-4 / IL4 human recombinant protein, 10 µg
Size:
10 ug 2 ug
  $300.00
2 Weeks*
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Other products for "IL4 or NM_000589"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 15 kDa
Purity >98% pure by SDS-PAGE and HPLC analyses.
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein.
Buffer System: PBS without stabilizer.
Bioactivity Biological: Recombinant Human IL-4 is fully biologically active when compared to standards.
The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is 0.1-0.3 ng/ml.
For most in vitro applications, IL-4 exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml.
Specific: 5 x 106 units/mg
Endotoxin < 0.1 ng per µg (IEU/µg) of IL-4
Reconstitution Method The lyophilized IL-4 is soluble in water and most aqueous buffers.
Restore in water to a concentration of 100 ng/ml.
This solution can be diluted into water or other buffered solutions or stored at -20°C for future use.
Preparation Lyophilized purified protein.
Protein Description Recombinant Human IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain having a molecular mass of 14.9 kDa and containing 129 amino acid residues.
Note Centrifuge vial before opening!
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_000580
Locus ID 3565
UniProt ID P05112
Cytogenetics 5q31.1
Synonyms IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
Summary The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. provided by RefSeq, Aug 2020
Protein Categories Allergies and autoimmune diseases, Cytokines, Growth Factors, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway
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Documents
Resources

file-alt Citations

Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response
Gómez-Fernández, Urtasun, Paton et al
Front Immunol (2018) 9, 2934 DOI: 10.3389/fimmu.2018.02934
AI Summary: OriGene contributed IL22RA2v2 myc-FLAG tag plasmid (RC219095) and vectors expressing C-terminal Myc-FLAG-tagged IL22RA2v2, IL-2, IL-4, IL-10, IL-17, IL-22, IFN-γ, and GRP94, which were used to study the secretion, function, and chaperone interactions of IL-22BP isoforms in this research.
Snippet: IL22RA2v2 myc-FLAG tag and IL22RA2v3-FLAG tag plasmids were, respectively purchased from Origene (RC219095) and GenScript (Ohu00490). …Vectors expressing C-terminal Myc-FLAG-tagged IL22RA2v2, IL-2, IL-4, IL-10, IL-17, IL-22, IFN-γ, and GRP94 were from Origene
FigureFigureFigure
Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival
Lee, Salazar, Joseph et al
Lab. Invest. (2018)
AI Summary: OriGene's recombinant OPNa, OPNb, and OPNc proteins were used as stimuli in cell migration assays to assess the differential effects of human osteopontin isoforms on macrophage migration in this study.
Snippet: Cells were allowed to migrate for 2 h in response to the following stimuli diluted in 0.1% FBS-Dulbecco's modified Eagle's medium: 100 ng/mL monocyte chemoattractant protein-1 (MCP-1), and 50 ng/mL of OPNa, OPNb, or OPNc recombinant protein (Origene)
An AAV Vector-Mediated Gene Delivery Approach Facilitates Reconstitution of Functional Human CD8(+) T Cells in Mice
Huang, Li, Coelho-Dos-Reis et al
PLoS ONE (2014) 9 (2), e88205 DOI: 10.1371/journal.pone.0088205
Cited by 49
AI Summary: OriGene products contributed to this study by providing the human GM-CSF, IL-3, IL-4, IL-7, and IL-15 cDNA used for AAV vector-mediated gene delivery to enhance human CD8(+) T cell reconstitution in mice.
Snippet: from A2-Tg NSG mice using PCR. HLA-A2.1 is herein designated HLA-A2 or A2 throughout the text. Human GM-CSF, IL-3, IL-4, IL-7, and IL-15 cDNA were purchased from OriGene. Human IL-15 was modified by replacing with ... Cite SaveSaving
FigureFigureFigure
Showing 1-3 of 3 papers.
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