Interleukin-3 / IL3 Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 15 kDa |
| Purity | >98% pure by SDS-PAGE & silver stain |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS Stabilizer: None |
| Bioactivity | Biological: Recombinant human IL-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1-0.5 ng/ml. Specific: 1 x 107 units/mg |
| Endotoxin | < 0.1 ng per µg (IEU/µg) of rh IL-3. |
| Reconstitution Method | Restore in water to a concentration not less than of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. For most in vitro applications, IL-3 exerts its biological activity in the concentration range of 0.1 to 1.0 ng/ml. |
| Preparation | Lyophilized purified protein |
| Protein Description | Recombinant Human IL-3 produced in E. coli is a 15.0 kDa globular protein containing 133 amino acid residues. |
| Note | Centrifuge vial before opening! |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_000579 |
| Locus ID | 3562 |
| UniProt ID | P08700 |
| Cytogenetics | 5q31.1 |
| Synonyms | IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF |
| Summary | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. provided by RefSeq, Jul 2008 |
| Protein Categories | Cytokines, Growth Factors, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Citations
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