Interleukin-3 / IL3 Human Protein

SKU DA3545

Interleukin-3 / IL3 human recombinant protein, 10 µg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

  N-terminal Sequence: MAPMTQT
• Predicted MW: 15 kDa
• Purity: >98% pure by SDS-PAGE & silver stain
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized purified protein; Buffer System: PBS; Stabilizer: None
• Bioactivity: Biological: Recombinant human IL-3 is fully biologically active when compared to standards. ; The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1-0.5 ng/ml.; Specific: 1 x 10⁷ units/mg
• Endotoxin: < 0.1 ng per µg (IEU/µg) of rh IL-3.
• Reconstitution Method: Restore in water to a concentration not less than of 0.1 mg/ml. ; This solution can be diluted into other buffered solutions or stored at -20°C for future use. ; For most in vitro applications, IL-3 exerts its biological activity in the concentration range of 0.1 to 1.0 ng/ml.
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant Human IL-3 produced in E. coli is a 15.0 kDa globular protein containing 133 amino acid residues.
• Note: Centrifuge vial before opening!
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient

Reference Data

• RefSeq: NP_000579
• Locus ID: 3562
• UniProt ID: P08700
• Cytogenetics: 5q31.1
• Synonyms: IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF
• Summary: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway