Interleukin-3 / IL3 Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Predicted MW | 15 kDa |
| Purity | >98% pure by SDS-PAGE & silver stain |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS Stabilizer: None |
| Bioactivity | Biological: Recombinant human IL-3 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1-0.5 ng/ml. Specific: 1 x 107 units/mg |
| Endotoxin | < 0.1 ng per µg (IEU/µg) of rh IL-3. |
| Reconstitution Method | Restore in water to a concentration not less than of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. For most in vitro applications, IL-3 exerts its biological activity in the concentration range of 0.1 to 1.0 ng/ml. |
| Preparation | Lyophilized purified protein |
| Protein Description | Recombinant Human IL-3 produced in E. coli is a 15.0 kDa globular protein containing 133 amino acid residues. |
| Note | Centrifuge vial before opening! |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_000579 |
| Locus ID | 3562 |
| UniProt ID | P08700 |
| Cytogenetics | 5q31.1 |
| Synonyms | IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF |
| Summary | The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. provided by RefSeq, Jul 2008 |
| Protein Categories | Cytokines, Growth Factors, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Amino acid starvation sensing dampens IL-1β production by activating riboclustering and autophagy
Battu, Afroz, Giddaluru et al
PLoS Biol. (2018) 16 (4), e2005317 DOI: 10.1371/journal.pbio.2005317
Battu, Afroz, Giddaluru et al
PLoS Biol. (2018) 16 (4), e2005317 DOI: 10.1371/journal.pbio.2005317
AI Summary: OriGene’s pCMV6 IL-1β plasmid was used in this study to investigate the regulation of IL-1β production under amino acid starvation conditions.
Snippet: ml. Rapamycin (Sigma-Aldrich) was used at a concentration of 200 nM. Lipofectamine 3000 was from Life Technologies (L3000015) and Sepharose G beads from Sigma-Aldrich. pCMV6 IL-1β was purchased from Origene. Generation of murine BMDMs Bone marrow from femurs and tibiae of C57BL/6J mice were flushed with ice-cold PBS/RPMI and cultured at a density of 5 × 106 to 7 × 106 cells in 10-cm Petri dish
An AAV Vector-Mediated Gene Delivery Approach Facilitates Reconstitution of Functional Human CD8(+) T Cells in Mice
Huang, Li, Coelho-Dos-Reis et al
PLoS ONE (2014) 9 (2), e88205 DOI: 10.1371/journal.pone.0088205
Huang, Li, Coelho-Dos-Reis et al
PLoS ONE (2014) 9 (2), e88205 DOI: 10.1371/journal.pone.0088205
Cited by 49
AI Summary: OriGene products contributed to this study by providing the human GM-CSF, IL-3, IL-4, IL-7, and IL-15 cDNA used for AAV vector-mediated gene delivery to enhance human CD8(+) T cell reconstitution in mice.
Snippet: from A2-Tg NSG mice using PCR. HLA-A2.1 is herein designated HLA-A2 or A2 throughout the text. Human GM-CSF, IL-3, IL-4, IL-7, and IL-15 cDNA were purchased from OriGene. Human IL-15 was modified by replacing with ... Cite SaveSaving
Showing 1-2 of 2 papers.
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