Flt3 ligand Human Protein
|Description||Flt3 ligand human protein, 10 µg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGG LWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP P SCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSS TLP PPWSPRPLEA TAPTA|
|Predicted MW||17.6 kDa|
|Purity||>98% by RP-HPLC, Anion-exchange FPLC, Silverstain|
|Buffer||Presentation State: Purified
State: Lyophilized (freeze-dried) without stabilizer
|Bioactivity||Biological: Human rh Flt3-ligand is fully biologically active when compared to standards.
The ED50 range is 0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of Human OCMI-AML5 cells.
|Endotoxin||< 0.1 ng per µg (IEU/µg) of rh Flt3-Ligand|
|Reconstitution||Recombinant Flt3-Ligand is soluble in water and most aqueous buffers.
Restore in water to a concentration not less than 0.1 mg/ml.
This solution can then be stored at 2-8°C for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
|Preparation||Lyophilized (freeze-dried) without stabilizer|
|Protein Description||Flt3-ligand produced in E. coli is a soluble non-glycosylated 17.6 kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||FL; FLG3L; FLT3L|
|Summary||Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane|
|Protein Pathways||Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer|
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