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Tumor necrosis factor (TNF-alpha) Human Protein

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SKU
DA3542X
Tumor necrosis factor (TNF-alpha) human recombinant protein, 50 µg
Size:
50 ug
  $300.00
2 Weeks*
Bulk/Customize

Other products for "TNF or NM_000594"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Predicted MW 17.48 kDa
Purity >98% by RP-HPLC, Anion-exchange FPLC, Silver stain
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized purified fraction
Buffer System: 20 mM PBS, pH 7.2, 10 mM NaCl
Bioactivity Biological: Human rh TNF-alpha is fully biologically active when compared to standards.
The ED50 as determined by the cytolysis of Murine L929 cells in the presence of Actinomycin-D is < 0.05 ng/ml.
Specific: 2 x 107 units/mg
Endotoxin < 0.1 ng per µg (IEU/µg) of rh TNF-alpha
Reconstitution Method Restore in sterile water to a concentration of 0.1 mg/ml.
This solution can be diluted into other buffered solutions or stored at –20°C for future use.
For most in-vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/ml.
Preparation Lyophilized purified fraction
Protein Description Recombinant Human TNF-a produced in E. coli is a single, non-glycosylated polypeptide having a Molecular Mass of 17.4 kDa protein and consisting of 158 amino acid residues.
Storage Store lyophilized rh TNF-alpha desiccated at -20°C.
Reconstituted rh TNF-alpha should be stored in working aliquots at -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_000585
Locus ID 7124
UniProt ID P01375
Cytogenetics 6p21.33
Synonyms TNF, TNF-a, TNFA, TNFSF2, Cachectin
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine. provided by RefSeq, Aug 2020
Protein Categories ADC Targets, Allergies and autoimmune diseases, Chemokines, Cytokines, Growth Factors, Intracellular Proteins, Secreated Proteins
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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