Tumor necrosis factor (TNF-alpha) Human Protein

CAT#: DA3542X

Reviews ()
Write a review

Tumor necrosis factor (TNF-alpha) human recombinant protein, 50 µg



USD 240.00


Availability*
2 Weeks

Size
    • 50 ug


Product images

Specifications

Product Data
Description Tumor necrosis factor (TNF-alpha) human recombinant protein, 50 µg
Species Human
Expression Host E. coli
Predicted MW 17.48 kDa
Purity >98% by RP-HPLC, Anion-exchange FPLC, Silver stain
Buffer Presentation State: Purified
State: Lyophilized purified fraction
Buffer System: 20mM PBS, pH 7.2, 10mM NaCl
Bioactivity Biological: Human rh TNF-alpha is fully biologically active when compared to standards.
The ED50 as determined by the cytolysis of Murine L929 cells in the presence of Actinomycin-D is < 0.05 ng/ml.
Specific: 2 x 107 units/mg
Endotoxin < 0.1 ng per µg (IEU/µg) of rh TNF-alpha
Reconstitution Restore in sterile water to a concentration of 0.1 mg/ml.
This solution can be diluted into other buffered solutions or stored at –20°C for future use.
For most in-vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/ml.
Preparation Lyophilized purified fraction
Protein Description Recombinant Human TNF-a produced in E. coli is a single, non-glycosylated polypeptide having a Molecular Mass of 17.4 kDa protein and consisting of 158 amino acid residues.
Storage Store lyophilized rh TNF-alpha desiccated at -20°C.
Reconstituted rh TNF-alpha should be stored in working aliquots at -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Avoid repeated freezing and thawing.
Stability Shelf life: One year from despatch.
Reference Data
RefSeq NP_000585
Locus ID 7124
Cytogenetics 6p21.33
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

Documents

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones
Molbio tools