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CD309 / VEGFR-2 / Flk-1 (D1-D7) Human Protein

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SKU
DA3530
CD309 / VEGFR-2 / Flk-1 (D1-D7) human recombinant protein, 5 µg
Size:
5 ug
  $205.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host Insect
Expression cDNA Clone or AA Sequence
Protein Sequence
ASVGLPSVSL DLPRLSIQKD ILTIKANTTL QITCRGQRDL DWLWPNNQSG SEQRVEVTEC SDGLFCKTLT IPKVIGNDTG AYKCFYRETD LASVIYVYVQ DYRSPFIASV SDQHGVVYIT ENKNKTVVIP CLGSISNLNV SLCARYPEKR FVPDGNRISW DSKKGFTIPS YMISYAGMVF CEAKINDESY QSIMYIVVVV GYRIYDVVLS PSHGIELSVG EKLVLNCTAR TELNVGIDFN WEYPSSKHQH KKLVNRDLKT QSGSEMKKFL STLTIDGVTR SDQGLYTCAA SSGLMTKKNS TFVRVHEKPF VAFGSGMESL VEATVGERVR IPAKYLGYPP PEIKWYKNGI PLESNHTIKA GHVLTIMEVS ERDTGNYTVI LTNPISKEKQ SHVVSLVVYV PPQIGEKSLI SPVDSYQYGT TQTLTCTVYA IPPPHHIHWY WQLEEECANE PSQAVSVTNP YPCEEWRSVE DFQGGNKIEV NKNQFALIEG KNKTVSTLVI QAANVSALYK CEAVNKVGRG ERVISFHVTR GPEITLQPDM QPTEQESVSL WCTADRSTFE NLTWYKLGPQ PLPIHVGELP TPVCKNLDTL WKLNATMFSN STNDILIMEL KNASLQDQGD YVCLAQDRKT KKRHCVVRQL TVLERVAPTI TGNLENQTTS IGESIEVSCT ASGNPPPQIM WFKDNETLVE DSGIVLKDGN RNLTIRRVRK EDEGLYTCQA CSVLGCAKVE AFFIIEGA
Predicted MW 116 kDa
Purity >95% pure as by SDS-PAGE and silver stain.
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized protein
Buffer System: 25 mM MES, 150 mM NaCl, pH 5.5
Stabilizer: None
Bioactivity Biological: Measured by its ability to inhibit the VEGF165-induced proliferation in Human Umbilical Vein Endothelial (HUVE) cells.
Inhibition of VEGF165-induced proliferationof HUVECs byrecombinant sVEGFR-2/KDR (Cat.-No DA3530).
Endotoxin < 0.1 ng per µg of sKDR
Reconstitution Method The lyophilized Human sKDR is soluble in water and most aqueous buffers.
Restore in water or PBS to a concentration of not lower than 0.1 mg/ml.
Preparation Lyophilized protein
Protein Description Recombinant Human soluble Endothelial Growth Factor Receptor-2 (sKDR D1-7) is produced as a non-chimeric protein in a monomeric form.
The soluble receptor protein consists of all 7 extracellular domains, which contain all the information necessary for high affinity ligand binding.
The receptor monomers have a mass of approximately 116kDa.
Result by N-terminal sequencing: ASVGLPSVSL
Length: 738 amino acids.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Shipping Ambient
Reference Data
RefSeq NP_002244
Locus ID 3791
UniProt ID P35968
Cytogenetics 4q12
Synonyms VEGFR2, FLK1, KDR, VEGF Receptor 2
Summary Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. provided by RefSeq, May 2009
Protein Categories ADC Targets, Cardiovascular diseases, CD Markers, Enzyme: Kinases, Growth Factors, Membrane Proteins
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, VEGF signaling pathway
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Documents
Resources

file-alt Citations

Apatinib Mesylate Inhibits the Proliferation and Metastasis of Epithelioid Malignant Peritoneal Mesothelioma In Vitro and In Vivo
Yang, Chen, Du et al
Front Oncol (2020) 10, 585079 DOI: 10.3389/fonc.2020.585079
Cited by 11
AI Summary: OriGene’s mouse anti-human Cytokeratin 5/6 antibody (OTI1C7) and mouse anti-human Ki-67 antibody (UMAB107) were used in this study to assess tumor cell characteristics and proliferation in evaluating the effects of apatinib on malignant peritoneal mesothelioma.
Snippet: mouse anti-human Cytokeratin 5/6 antibody (OriGene, OTI1C7), and mouse anti-human Ki-67 antibody (OriGene, UMAB107)
FigureFigureFigure
IHC
Complex Formation between VEGFR2 and the β2-Adrenoceptor
Kilpatrick, Alcobia, White et al
Cell Chem Biol (2019) 26 (6), 830-841.e9 DOI: 10.1016/j.chembiol.2019.02.014
AI Summary: OriGene contributed a VEGFR2 plasmid (GenBank: NM_002253) that was used to generate NLuc-VEGFR2 and HaloTag-VEGFR2 constructs for studying protein-protein interactions in this paper.
Snippet: … Briefly, VEGFR2 was subcloned from a plasmid obtained from Origene (GenBank: NM_002253;Maryland, USA). …Briefly, VEGFR2 was subcloned from a plasmid obtained from Origene (GenBank: NM_002253;;NLuc-VEGFR2 and HaloTag-VEGFR2 constructs were generated as described in Kilpatrick et al., 2017). Briefly, VEGFR2 was subcloned from a plasmid obtained from Origene (GenBank: NM_002253;Promega Corporation, USA) encoding a fusion of the signal peptide sequence of IL-6 onto the N terminus of NanoLuc (NLuc). …
Apatinib, an Inhibitor of Vascular Endothelial Growth Factor Receptor 2, Suppresses Pathologic Ocular Neovascularization in Mice
Kim, Suh
Invest. Ophthalmol. Vis. Sci. (2017) 58 (9), 3592-3599 DOI: 10.1167/iovs.17-21416
AI Summary: OriGene contributed to this study by providing the phospho-VEGFR2 (p-VEGFR2) antibody, which was used in Western blot experiments to assess VEGFR2 phosphorylation and evaluate the inhibitory effects of apatinib on VEGF signaling in ocular neovascularization models.
Snippet: The blots were incubated with the appropriate primary IgGs: phospho-VEGFR2 (p-VEGFR2;Origene, Rockville, MD, USA), phospho-extracellular signal-regulated kinase (p-ERK;R&D Systems, Minneapolis, MN, USA), VEGFR2 (R&D Systems), ERK (Cell Signaling ... All 3 versions Cite SaveSaving...;... The blots were incubated with the appropriate primary IgGs: phospho-VEGFR2 (p-VEGFR2;R&D Systems, Minneapolis, ...
WB IF
CD133(+) cancer stem cells promoted by VEGF accelerate the recurrence of hepatocellular carcinoma
Liu, Hao, Ouyang et al
Sci Rep (2017) 7, 41499 DOI: 10.1038/srep41499
AI Summary: OriGene products contributed to this study by providing lentiviral shRNA constructs targeting Nanog and VEGFR2, which were used to investigate the roles of these genes in cancer stem cell function and hepatocellular carcinoma recurrence.
Snippet: described 16 . Briefly, cells were transfected with lentiviral constructs expressing shRNAs for Nanog, VEGFR2 (OriGene, MD, USA) or sh Ctrl for 24 hours. Positive cells were selected with puromycin for 7 days. Recombinant ... All 5 versions Cite SaveSaving
FigureFigureFigure
miR-24 Inhibition Increases Menin Expression and Decreases Cholangiocarcinoma Proliferation
Ehrlich, Hall, Venter et al
Am. J. Pathol. (2017)
AI Summary: OriGene contributed to this study by providing the human pCMV6-Entry MEN1 cDNA and control vector, which were used to manipulate menin expression in cholangiocarcinoma cell lines.
Snippet: Biotechnology). Human pCMV6-Entry MEN1 cDNA and control vector were purchased from Origene (Rockville, MD) and transfected with Lipofectamine 2000 Transfection reagent (ThermoFisher Scientific, Grand Island, NY).
Acidic Fibroblast Growth Factor Promotes Endothelial Progenitor Cells Function via Akt/FOXO3a Pathway
Huang, Wang, Wang et al
PLoS ONE (2015) 10 (6), e0129665 DOI: 10.1371/journal.pone.0129665
Cited by 13
AI Summary: In this study, the CD133 antibody from OriGene was used to immunofluorescently identify endothelial progenitor cells, enabling the researchers to assess the effects of FGF1 on EPC function.
Snippet: experiments. For EPC identification, cells were immunofluorescently stained using antibodies against endothelial markers CD34 (Epitomics) and VEGFR-2 (R&D Systems) as well as stem/progenitor marker CD133 (Origene). ... Cite SaveSaving
FigureFigureFigure
Showing 1-6 of 7 papers.
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