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Hepatocyte growth factor / HGF Human Protein

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SKU
DA3506
Hepatocyte growth factor / HGF human recombinant protein, 5 µg
Size:
5 ug
  $310.00
2 Weeks*
Bulk/Customize

Other products for "HGF or NM_000601"

Specifications
Specifications
Product Data
Species Human
Expression Host Insect
Predicted MW 78 kDa
Concentration N/A
Purity >95% pure by SDS-PAGE and visualised by silver stain.
Presentation State Purified
Buffer Presentation State: Purified
State: Lyophilized without preservatives.
Buffer System: 50 mM Acetic Acid with BSA as stabilizer.
Bioactivity Biological: Human HGF is fully biologically active when compared to standards.
The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 0.5-1.0 ng/ml.
Endotoxin < 0.1 ng per µg of HGF
Reconstitution Method The lyophilized Human HGF is soluble in Acetic Acid (50 mM) and can be restored to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
Preparation Lyophilized without preservatives.
Protein Description Recombinant human HGF is a 78.0 kDa protein.
Storage The lyophilized protein is stable at -20°C for one year from despatch.
Reconstituted human HGF is stable for three months when stored in working aliquots
at -20°C.
Avoid repeated freezing and thawing.
Shipping Ambient
Reference Data
RefSeq NP_000592
Locus ID 3082
UniProt ID P14210
Cytogenetics 7q21.11
Synonyms Scatter factor, Hepatopoeitin-A, HPTA
Summary This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. provided by RefSeq, Nov 2015
Protein Categories Cancer: Gastrointestinal, Cytokines, Enzyme: Peptidases, Growth Factors, Nervous system Diseases, Secreated Proteins
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma
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Documents
Resources

file-alt Citations

Insufficiency of hepatocyte growth factor activator inhibitor-1 confers lymphatic invasion of tongue carcinoma cells
Izumi, Yamamoto, Kawaguchi et al
Cancer Sci (2022) 113 (6), 2179-2193 DOI: 10.1111/cas.15346
Cited by 6
AI Summary: In this study, OriGene products—specifically the SPINT1 (HAI-1) knockout (KO) HSC3 and SAS cell lines—were used to investigate how loss of HAI-1 promotes lymphatic invasion and protease activity in tongue squamous cell carcinoma cells.
Snippet: lymph node metastasis associated with decreased HAI‐1 remains to be clarified. In this study, we examined the effects of HAI‐1 loss on the invasive growth of TSCC cells in vitro as well as on tumorigenesis, lymphatic invasion and lymph node metastasis in vivo using an orthotopic xenotransplantation method. Our results suggested that HAI‐1 deficiency induced dysregulated pericellular proteas
FigureFigureFigure
IHC
Production of human mutant biologically active hepatocyte growth factor in Chinese hamster ovary cells
Xu, Wan, Peng et al
Prep. Biochem. Biotechnol. (2017)
AI Summary: OriGene contributed to this study by providing the cDNA encoding human HGF, which was used as the template for generating the mutant HGF (R494E) expressed in CHO cells.
Snippet: Protein Expression and Purification. 2015, 113, 1-7 ] . The cDNA encoding human HGF was purchased from Origene and the replacement 494 R of E was done by fusion PCR (Table 1 Replacement of the amino acid codon marked underline).
Hepatocyte growth factor-modified adipose tissue-derived stem cells improve erectile function in streptozotocin-induced diabetic rats
Liu, Peng, Jia et al
Growth Factors (2015) 33 (4), 282-9 DOI: 10.3109/08977194.2015.1077825
Cited by 30
AI Summary: OriGene contributed to this study by providing the plasmid encoding human HGF, which was used to generate hepatocyte growth factor-modified adipose tissue-derived stem cells for investigating improved erectile function in diabetic rats.
Snippet: 2 and passaged using HyQtase (HyClone). Construction of recombinant lentiviral vectors. The plasmid encoding human HGF (hHGF) was purchased from OriGene (Rockville, MD). The lentiviral vector system (GeneChem, Shanghai ... Cite SaveSaving
Protein kinase C stimulates HuD-mediated mRNA stability and protein expression of neurotrophic factors and enhances dendritic maturation of hippocampal neurons in culture
Lim, Alkon
Hippocampus (2012) 22 (12), 2303-19 DOI: 10.1002/hipo.22048
Cited by 73
AI Summary: OriGene contributed gene-specific primers for BDNF, NGF, and NT-3, which were used in this study to measure neurotrophic factor mRNA levels and assess the effects of HuD and PKC signaling on their expression.
Snippet: NTFs, neurotrophic factors;PKC, protein kinase C;RIP, RNA-binding protein immunoprecipitation;shRNA, short hairpin RNA ... The following primers (Origene) were used: BDNF, 5′- GGCTGACACTTTTGAGCACGTC-3′ and 5′-CTCCAAAGGCACTTGACTGCTG-3′;nerve
Showing 1-4 of 4 papers.
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