GM-CSF (His-tag) Human Protein
GM-CSF (His-tag) human recombinant protein, 50 µg
|Description||GM-CSF (His-tag) human recombinant protein, 50 µg|
|Predicted MW||14.6 kDa|
|Purity||>98% pure by SDS-PAGE and visualised by silver stain.|
|Buffer||Presentation State: Purified
State: Lyophilized without preservatives and stabilizers
Buffer System: PBS
|Bioactivity||Biological: Human GM-CSF is fully biologically active when compared to standards.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol, 1989]. The ED50 for this effect is typically < 0.1ng/ml corresponding to a specific activity of ≥ 1 x 107 units/mg.
|Endotoxin||< 0.1 ng per µg of GM-CSF|
|Reconstitution||The lyophilized GM-CSF is soluble in water and most aqueous buffers.
The lyophilized powder can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
|Preparation||Lyophilized without preservatives and stabilizers|
|Protein Description||Recombinant human GM-CSF.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Summary||The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein|
|Protein Pathways||Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway|