GM-CSF (His-tag) Human Protein

CAT#: DA3505XD

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GM-CSF (His-tag) human recombinant protein, 50 µg


Product Datasheet for 'DA3505XD'


USD 515.00


Availability*
2 Weeks

Size
    • 50 ug


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Specifications

Product Data
Description GM-CSF (His-tag) human recombinant protein, 50 µg
Species Human
Expression Host Insect
Tag His-tag
Predicted MW 14.6 kDa
Purity >98% pure by SDS-PAGE and visualised by silver stain.
Buffer Presentation State: Purified
State: Lyophilized without preservatives and stabilizers
Buffer System: PBS
Bioactivity Biological: Human GM-CSF is fully biologically active when compared to standards.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol, 1989]. The ED50 for this effect is typically < 0.1ng/ml corresponding to a specific activity of ≥ 1 x 10units/mg.
Endotoxin < 0.1 ng per µg of GM-CSF
Reconstitution The lyophilized GM-CSF is soluble in water and most aqueous buffers.
The lyophilized powder can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
Preparation Lyophilized without preservatives and stabilizers
Protein Description Recombinant human GM-CSF.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000749
Locus ID 1437
Cytogenetics 5q31.1
Synonyms CSF; GMCSF
Summary The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

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