BMP2 / BMP2A Human Protein
BMP2 / BMP2A human recombinant protein, 25 µg
|Description||BMP2 / BMP2A human recombinant protein, 25 µg|
|Expression Host||E. coli|
|Predicted MW||26 kDa|
|Purity||>95% pure by SDS-PAGE and visualised by silver stain|
|Buffer||Presentation State: Purified
State: Lyophilized purified Ig fraction
Buffer System: 50mM Acetic Acid
|Bioactivity||Biological: Measured by the ability of BMP-2 to induce Alkaline Phosphatase production by C2C12 myogenic cells.
The ED50 for this effect is typically 0.3-0.8 µg/ml.
Specific: 2.5 x 103 units/mg
|Endotoxin||< 0.1 ng per µg of BMP-2|
|Reconstitution||The lyophilized BMP-2 is best soluble in 50 mM Acetic Acid and at a concentration of 0.1 mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0|
|Preparation||Lyophilized purified Ig fraction|
|Protein Description||Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein.
BMP-2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity.
The amino acid sequence of recombinant Human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector.
|Storage||Lyophilized samples are stable for 6 months from despatch at -20°C to -70°C.
Reconstituted BMP-2 should be stored in working aliquots at -20°C.
Avoid repeated freezing and thawing.
|Synonyms||BDA2; BMP2A; SSFSC|
|Summary||This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane|
|Protein Pathways||Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway|