BMP2 / BMP2A Human Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Predicted MW | 26 kDa |
| Purity | >95% pure by SDS-PAGE and visualised by silver stain |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Lyophilized purified Ig fraction Buffer System: 50 mM Acetic Acid Stabilizer: None |
| Bioactivity | Biological: Measured by the ability of BMP-2 to induce Alkaline Phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 µg/ml. Specific: 2.5 x 103 units/mg |
| Endotoxin | < 0.1 ng per µg of BMP-2 |
| Reconstitution Method | The lyophilized BMP-2 is best soluble in 50 mM Acetic Acid and at a concentration of 0.1 mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0 |
| Preparation | Lyophilized purified Ig fraction |
| Protein Description | Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein. BMP-2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity. The amino acid sequence of recombinant Human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector. |
| Storage | Lyophilized samples are stable for 6 months from despatch at -20°C to -70°C. Reconstituted BMP-2 should be stored in working aliquots at -20°C. Avoid repeated freezing and thawing. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_001191 |
| Locus ID | 650 |
| UniProt ID | P12643 |
| Cytogenetics | 20p12.3 |
| Synonyms | Bone morphogenetic protein 2, BMP-2, BMP-2A |
| Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. provided by RefSeq, Jul 2016 |
| Protein Categories | Cytokines, Growth Factors, Secreated Proteins |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane |
| Protein Pathways | Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Citations
Development of novel gene carrier using modified nano hydroxyapatite derived from equine bone for osteogenic differentiation of dental pulp stem cells
Lee, Seonwoo, Jang et al
Bioact Mater (2021) 6 (9), 2742-2751 DOI: 10.1016/j.bioactmat.2021.01.020
Lee, Seonwoo, Jang et al
Bioact Mater (2021) 6 (9), 2742-2751 DOI: 10.1016/j.bioactmat.2021.01.020
Cited by 29
AI Summary: In this study, OriGene’s BMP-2 plasmid and RUNX2, OCN, and OPN primers were used to evaluate and confirm the effectiveness of the newly developed gene carrier in promoting osteogenic differentiation of dental pulp stem cells.
Non-viral gene delivery embedded in biomimetically mineralized matrices for bone tissue engineering
Acri, Laird, Jaidev et al
Tissue Eng Part A (2020)
Acri, Laird, Jaidev et al
Tissue Eng Part A (2020)
AI Summary: OriGene contributed pFGF-2 plasmid (No. SC118884) and pBMP-2 plasmid (No. SC119392) to this study, enabling the investigation of growth factor gene delivery for enhanced bone regeneration in biomimetically mineralized matrices.
Snippet: 13031 open read frame [ORF] ref LC337019.1;Addgene, Watertown, MA) and the pFGF-2 and pBMP-2 were purchased from OriGene (Rockville, MD;FGF-2 plasmid No. SC118884 ORF ref NM_002006.3, BMP-2 plasmid No;- plasmid #13031 ORF ref LC337019.1) and the pFGF-2 and pBMP-2 were purchased from OriGene (Rockville, MD, FGF-2 plasmid #SC118884 ORF ref NM_002006.3, BMP-2 plasmid #SC119392 ORF ref NM_001200.1). …
Nanofibrous Scaffolds Containing Hydroxyapatite and Microfluidic-Prepared Polyamidoamin/BMP-2 Plasmid Dendriplexes for Bone Tissue Engineering Applications
Doosti-Telgerd, Mahdavi, Moradikhah et al
Int J Nanomedicine (2020) 15, 2633-2646 DOI: 10.2147/IJN.S244416
Doosti-Telgerd, Mahdavi, Moradikhah et al
Int J Nanomedicine (2020) 15, 2633-2646 DOI: 10.2147/IJN.S244416
Cited by 1
AI Summary: OriGene’s pCMV6-AC-GFP plasmid was used as the source of BMP-2 DNA for gene delivery experiments in the study, enabling the fabrication of scaffolds for bone tissue engineering applications.
Snippet: Materials and Methods. Plasmid DNA Preparation and Extraction. Plasmid DNA (pCMV6-AC-GFP, OriGene) containing BMP-2 sequence were amplified in Escherichia coli hot strain, DH5α, grown overnight in Luria-Broth base medium containing ampicillin
Two novel BMP-2 variants identified in patients with thoracic ossification of the ligamentum flavum
Qu, Chen, Fan et al
Eur. J. Hum. Genet. (2017)
Qu, Chen, Fan et al
Eur. J. Hum. Genet. (2017)
AI Summary: OriGene’s pCMV6-Entry vector carrying human BMP-2 cDNA ORF with a C-terminal myc-DDK-tag was used in this study to express wild-type BMP-2 for functional assays investigating the impact of novel BMP-2 variants in patients with thoracic ossification of the ligamentum flavum.
Snippet: Plasmid transfection and western blot analysis. The pCMV6-Entry vector carrying the human BMP-2 cDNA ORF and the C-terminal myc-DDK-tag (referred to as WT) was purchased from OriGene Technologies (Beijing, China). ... All 3 versions Cite SaveSaving
Showing 1-4 of 4 papers.
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