HCV NS4 (1658-1863, 19 kDa) Protein
Specifications
| Product Data | |
| Expression Host | E. coli |
|---|---|
| Concentration | lot specific |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Liquid purified Ig fraction (95% pure by SDS-PAGE and Bradford et al). Buffer System: 8 M urea, 20 mM Tris-HCl, pH 8.0; 10 mM beta-mercaptoethanol. without preservatives. |
| Preparation | Liquid purified Ig fraction (95% pure by SDS-PAGE and Bradford et al). |
| Recommended Dilution | Suitable in Western Blot with a suggested dilution of 1:1,000. |
| Protein Description | HCV NS4 antigen (recombinant) NS4a+b a.a 1658 to a.a 1863 of HCV polyprotein, 19 kDa, denatured. Beta-galactosidase (114 kDa) fused at N-terminus. Reacts strongly with human HCV positive serum. |
| Storage | Store the antigen at 2-8°C. DO NOT FREEZE!! |
| Stability | Shelf life: six months from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Hepatitis C Virus, NS4B, Non-structural protein NS4A |
| Summary | Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. |
| Protein Families | 000., Suitable in Western Blot with a suggested dilution of 1:1 |
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Resources
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