HCV NS3 (1450-1643, 22kDa) Protein
Specifications
| Product Data | |
| Expression Host | E. coli |
|---|---|
| Concentration | lot specific |
| Purity | >95% |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Liquid purified fraction (>90% pure by SDS-PAGE and Bradford et. al.). Buffer System: 8 M Urea, 20 mM Tris-HCl pH 8.0 containing 200 mM Imidazole without preservatives. |
| Preparation | Liquid purified fraction (>90% pure by SDS-PAGE and Bradford et. al.). |
| Recommended Dilution | Suitable in Western Blot with a suggested dilution of 1:1,000. |
| Protein Description | HCV NS3 antigen (recombinant) a.a 1450 to a.a 1643 of HCV polyprotein, 22kDa. Six Histidine fusion at the carboxy-terminal end. Reactivity with human HCV positive serum undetermined. |
| Storage | Store the antigen at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid multiple freeze/thaw cycles. |
| Stability | Shelf life: six months from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | Hepatitis C Virus Serine proteiase NS3 |
| Summary | Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. |
| Protein Families | 000., Suitable in Western Blot with a suggested dilution of 1:1 |
Reviews
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Resources
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