Hepatitis B Surface Antigen / HBsAg (adw) Protein
Specifications
| Product Data | |
| Expression Host | S. cerevisiae |
|---|---|
| Concentration | lot specific |
| Purity | >98% pure by SDS-PAGE. Purification is conducted from broken yeast cells by the following scheme: Clarification, Microfiltration, Ultrafiltration, Adsorption Chromatography, Ion-exchange chromatography, Ultracentrifugation and Sterile Filtration |
| Presentation State | Purified |
| Buffer | Presentation State: Purified State: Liquid (sterile filtered) purified protein Buffer System: 0.05M Phosphate, 0.2M Sodium Chloride, pH 7.2 without preservatives |
| Preparation | Liquid (sterile filtered) purified protein |
| Recommended Dilution | Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies. |
| Protein Description | Recombinant Hepatitis B Surface Antigen (HBsAg), (adw) (S region) is a 24 kDa protein containing 227 amino acids, comprising the full-length of the S-gene. Does not contain a fusion partner. |
| Storage | Store the antigen at 2-8°C. DO NOT FREEZE!! |
| Stability | Shelf life: six months from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
| Synonyms | HBV surface antigen, Hepatitis B Virus |
| Summary | Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction. |
| Protein Families | Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies. |
Reviews
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Resources
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