Vimentin Human Protein

CAT#: BA1017S

Vimentin human recombinant protein, 0.1 mg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 54 kDa
• Concentration: 1.0 mg/ml (after reconstitution)
• Purity: >95% pure determined by SDS Gel Electrophoresis
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized protein
  Buffer System: Final solution contains 30 mM Tris/HCI pH 8, 9.5M Urea, 2 mM DTT, 2 mM EDTA, 10 mM Methylammonium Chloride
• Reconstitution: Restore with 70 µl distilled water (final volume 100 µl).
• Preparation: Lyophilized protein
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
  Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4).
  For immunization purposes, the solution can be further dialyzed against PBS (Phosphate Buffered Saline, e.g. Dulbecco's PBS).
• Protein Description: Standard Recombinant Human Vimentin.
• Note: Isoelectric Point: pI 5.3
• Storage: Store at 2-8°C (lyophilized) or at -20°C (reconstituted).
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_003371
• Locus ID: 7431
• UniProt ID: P08670, V9HWE1
• Cytogenetics: 10p13
• Summary: This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
• Protein Families: ES Cell Differentiation/IPS